The adsorption behaviour of methylcellulose on talc surface and the stimulus response of methylcellulose on talc flotation were studied through flotation tests,adsorption experiments and zeta potential measurements.. The results show that methylcellulose dissolved in water and hydrophobic interaction is the main driving force for adsorption at the low temperature. When the temperature increases, methylcellulose precipitation from solution and deposited on talc surface, the adsorption amount is large and the depression effect is strong. The depression effect of adsorbed methylcellulose layer on talc flotation is also affected by temperature. The switch of temperature from low to high results in decreased depression effect of methylcellulose and the change is reversible. The reason is that the hydroxyl of methylcellulose form hydrogen bonds with water molecules at low temperature, leading to a hydrophilic mineral surface and strong depression effect on talc. When the temperature rises, methylcellulose molecules lose water and the water content decreases, the depression effect to talc also decreases.