色谱 , 2017, 35(1): 105-109.
10.3724/SP.J.1123.2016.08039
单细胞分析可以揭示生命基本单元———细胞物质组成、生理行为的多样性和差异性，是当今生命分析的主流前沿技术。同时，也对分析技术的定量检测极限、分辨率和精密操控能力提出了新挑战。文章着重从单细胞分离、检测、成像等几个主要方面，综述了单细胞技术研究的最新进展，并对分离和检测技术的发展作了展望。
参考文献：
[1] Kleparnik K;Horky M.Detection of DNA fragmentation in a single apoptotic cardiomyocyte by electrophoresis on a microfluidic device.[J].Electrophoresis: The Official Journal of the International Electrophoresis Society,200321(21):3778-3783.
[2] Huang B;Wu HK;Bhaya D;Grossman A;Granier S;Kobilka BK;Zare RN.Counting low-copy number proteins in a single cell[J].Science,200726(26):81-84.
[3] Marc PJ;Sims CE;Bachman M;Li GP;Allbritton NL.Fast-lysis cell traps for chemical cytometry[J].Lab on a chip,20085(5):710-716.
[4] Deng, B.;Wang, Z.;Song, J.;Xiao, Y.;Chen, D.;Huang, J..Analysis of doxorubicin uptake in single human leukemia K562 cells using capillary electrophoresis coupled with laser-induced fluorescence detection[J].Analytical and bioanalytical chemistry,20117(7):2143-2152.
[5] Huang B;Wang W;Bates M;Zhuang X.Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy.[J].Science,20085864(5864):810-813.
[6] Xu, K.;Babcock, H.P.;Zhuang, X..Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton[J].Nature methods,20122(2):185-188.
[7] Konorov SO;Glover CH;Piret JM;Bryan J;Schulze HG;Blades MW;Turner RFB.In situ analysis of living embryonic stem cells by coherent anti-stokes Raman Microscopy[J].Analytical chemistry,200718(18):7221-7225.
[8] Fu, D.;Lu, F.-K.;Zhang, X.;Freudiger, C.;Pernik, D.R.;Holtom, G.;Xie, X.S..Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy[J].Journal of the American Chemical Society,20128(8):3623-3626.
材料期刊网