色谱 , 2017, 35(3): 229-236.
10.3724/SP.J.1123.2016.10040
蛋白质的末端不仅是蛋白质合成的起点和终点,更参与并调节蛋白质的各项生理功能.研究蛋白质的末端不仅对于蛋白质的结构和功能注释十分必要,还为揭示蛋白酶的作用机理提供重要信息.该文着重针对近年来蛋白质组末端肽富集策略方面取得的进展进行综述,并对蛋白质末端组学技术在蛋白酶作用底物和位点分析中的应用进行了概述.此外,还对蛋白质末端组学研究的发展前景进行了展望.
参考文献：
[1] Chung JJ;Shikano S;Hanyu Y;Li M.Functional diversity of protein C-termini: more than zipcoding? [Review][J].Trends in Cell Biology,20023(3):146-150.
[2] Nakazawa T;Yamaguchi M;Okamura TA;Ando E;Nishimura O;Tsunasawa S.Terminal proteomics: N- and C-terminal analyses for high-fidelity identification of proteins using MS.[J].Proteomics,20084(4):673-685.
[3] McDonald L;Robertson DHL;Hurst JL;Beynon RJ.Positional proteomics: selective recovery and analysis of N-terminal proteotytic peptides[J].Nature methods,200512(12):955-957.
[4] Edwards RJ;Wrigley A;Bai Z;Bateman M;Russell H;Murray S;Lu H;Taylor GW;Boobis AR;Sriskandan S.C-Terminal antibodies (CTAbs): A simple and broadly applicable approach for the rapid generation of protein-specific antibodies with predefined specificity[J].Proteomics,20079(9):1364-1372.
[5] Yamaguchi M;Nakazawa T;Kuyama H;Obama T;Ando E;Okamura T;Ueyama N;Norioka S.High-throughput method for N-terminal sequencing of proteins by MALDI mass spectrometry[J].Analytical chemistry,20052(2):645-651.
[6] Mahrus S;Trinidad JC;Barkan DT;Sali A;Burlingame AL;Wells JA.Global Sequencing of proteolytic cleavage sites in apoptosis by specific Labeling of protein N termini[J].Cell,20085(5):866-876.
[7] Kuyama H;Sonomura K;Nishimura O;Tsunasawa S.A method for N-terminal de novo sequence analysis of proteins by matrix-assisted laser desorption/ionization mass spectrometry[J].Analytical Biochemistry: An International Journal of Analytical and Preparative Methods,20082(2):291-296.
[8] Gevaert K;Goethals M;Martens L;Van Damme J;Staes A;Thomas GR.Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides[J].Nature biotechnology,20035(5):566-569.
[9] Venne, A.S.;V?gtle, F.-N.;Meisinger, C.;Sickmann, A.;Zahedi, R.P..Novel highly sensitive, specific, and straightforward strategy for comprehensive N-terminal proteomics reveals unknown substrates of the mitochondrial peptidase Icp55[J].Journal of proteome research,20139(9):3823-3830.
[10] Kuyama H;Shima K;Sonomura K;Yamaguchi M;Ando E;Nishimura O;Sunasawa S.A simple and highly successful C-terminal sequence analysis of proteins by mass spectrometry[J].Proteomics,20088(8):1539-1550.
[11] Backes,BJ;Harris,JL;Leonetti,F;Craik,CS;Ellman,JA.Synthesis of positional-scanning libraries of fluorogenic peptide substrates to define the extended substrate specificity of plasmin and thrombin (see comments)[J].Nature biotechnology,20002(2):187-193.
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