A novel method of designing and preparing bone tissue engineering scaffolds with controllable porous structure of both macro channels and micro pores was proposed. The CAD soft-ware UG NX3.0 was used to design the macro channels' shape, size and distribution. By integrating rapid prototyping and traditional porogen technique, the macro channels and micro pores were formed respectively. The size, shape and quantity of micro pores were controlled by porogen particulates. The sintered β-TCP porous scaffolds possessed connective macro channels of approximately 500 μm and micro pores of 200-400 μm. The porosity and connectivity of micro pores became higher with the in-crease of porogen ratio, while the mechanical properties weakened. The average porosity and com-pressive strength of β-TCP scaffolds prepared with porogen ratio of 60wt% were 78.12% and 0.2983 Mpa,respectively. The cells' adhesion ratio of scaffolds was 67.43%. The ALP activity, OCN content and cells micro morphology indicated that cells grew and proliferated well on the scaffolds.
参考文献
| [1] | Woo KM;Jun JH;Chen VJ;Seo JY;Baek JH;Ryoo HM;Kim GS;Somerman MJ;Ma PX .Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization[J].Biomaterials,2007(26):335-343. |
| [2] | Freed,LE;Martin,I;Vunjak,Novakovic-G .Frontiers in tissue engineering. In vitro modulation of chondrogenesis.[J].Clinical Orthopaedics and Related Research,1999(367 Suppl):46-58. |
| [3] | Ivan Martin;David Wendt;Michael Heberer .The role of bioreactors in tissue engineering[J].Trends in Biotechnology,2004(2):80-86. |
| [4] | David Pazzano;Kathi A. Mercier;John M. Moran;Stephen S. Fong;David D. DiBiasio;Jill X. Rul .Comparison of chondrogensis in static and perfused bioreactor culture[J].Biotechnology Progress,2000(5):893-896. |
| [5] | Rose FR;Hou Q;Oreffo RO .Delivery systems for bone growth factors - the new players in skeletal regeneration.[J].Journal of Pharmacy and Pharmacology,2004(4):415-427. |
| [6] | Lin AS;Barrows TH;Cartmell SH;Guldberg RE .Microarchitectural and mechanical characterization of oriented porous polymer scaffolds.[J].Biomaterials,2003(3):481-489. |
| [7] | Chu TM;Orton DG;Hollister SJ;Feinberg SE;Halloran JW .Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures.[J].Biomaterials,2002(5):1283-1293. |
| [8] | Hollister SJ;Maddox RD;Taboas JM .Optimal design and fabrication of scaffolds to mimic tissue properties and satisfy biological constraints.[J].Biomaterials,2002(20):4095-4103. |
| [9] | Peter X. Ma;Ruiyun Zhang .Microtubular architecture of biodegradable polymer scaffolds[J].Journal of biomedical materials research, Part B. Applied biomaterials,2001(4):469-477. |
| [10] | Charriere E;Lemaitre J;Zysset P .Hydroxyapatite cement scaffolds with controlled macroporosity: fabrication protocol and mechanical properties.[J].Biomaterials,2003(5):809-817. |
| [11] | Rose FR;Cyster LA;Grant DM;Scotchford CA;Howdle SM;Shakesheff KM .In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel.[J].Biomaterials,2004(24):5507-5514. |
| [12] | Silva MMCG;Cyster LA;Barry JJA;Yang XB;Oreffo ROC;Grant DM;Scotchford CA;Howdle SM;Shakesheff KM;Rose FRAJ .The effect of anisotropic architecture on cell and tissue infiltration into tissue engineering scaffolds[J].Biomaterials,2006(35):5909-5917. |
| [13] | Vidaurre A;Cortazar IC;Ribelles JLG .Polymeric scaffolds with a double pore structure[J].Journal of Non-Crystalline Solids: A Journal Devoted to Oxide, Halide, Chalcogenide and Metallic Glasses, Amorphous Semiconductors, Non-Crystalline Films, Glass-Ceramics and Glassy Composites,2007(11/12):1095-1100. |
| [14] | 吴林波,丁建东.具有复杂形状的聚ε-己内酯多孔支架的模压制备方法[J].高分子材料科学与工程,2005(01):296-299. |
| [15] | 石浦江,李玉宝,张利,彭雪林,周钢,邹琴.载微球纳米羟基磷灰石/壳聚糖复合多孔支架的制备与表征[J].功能材料,2006(11):1798-1800,1804. |
| [16] | Deville S;Saiz E;Tomsia AP .Freeze casting of hydroxyapatite scaffolds for bone tissue engineering[J].Biomaterials,2006(32):5480-5489. |
| [17] | Madihally SV;Matthew HW .Porous chitosan scaffolds for tissue engineering.[J].Biomaterials,1999(12):1133-1142. |
| [18] | A. Woesz;M. Rumpler;J. Stampfl;F. Varga;N. Fratzl-Zelman;P. Roschger;K. Klaushofer;P. Fratzl .Towards bone replacement materials from calcium phosphates via rapid prototyping and ceramic gelcasting[J].Materials science & engineering, C. Biomimetic and supramolecular systems,2005(2):181-186. |
| [19] | 李祥,李涤尘,张彦东,卢秉恒.骨组织微结构观察分析及仿生支架立体光固化间接制造[J].北京生物医学工程,2006(02):164-167,192. |
| [20] | Williams JM;Adewunmi A;Schek RM;Flanagan CL;Krebsbach PH;Feinberg SE;Hollister SJ;Das S .Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering[J].Biomaterials,2005(23):4817-4827. |
| [21] | Tan KH.;Chua CK.;Leong KF.;Cheah CM.;Cheang P.;Abu Bakar MS.;Cha SW. .Scaffold development using selective laser sintering of polyetheretherketone-hydroxyapatite biocomposite blends[J].Biomaterials,2003(18):3115-3123. |
| [22] | Samar Jyoti Kalita;Susmita Bose;Howard L. Hosick;Amit Bandyopadhyay .Development of controlled porosity polymer-ceramic composite scaffolds via fused deposition modeling[J].Materials science & engineering, C. Biomimetic and supramolecular systems,2003(5):611-620. |
| [23] | Zein I;Hutmacher DW;Tan KC;Teoh SH .Fused deposition modeling of novel scaffold architectures for tissue engineering applications.[J].Biomaterials,2002(4):1169-1185. |
| [24] | Lee M;Dunn JC;Wu BM .Scaffold fabrication by indirect three-dimensional printing.[J].Biomaterials,2005(20):4281-4289. |
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