In order to study the biocompatibility of self-assembled FGL peptide nanofibers scaffold with neural stem cells(NSCs),FGL pepitide-amphiphile(FGL-PA)was synthesized by solid-phase peptide synthesis technique.The diluted hydrochloric acid was added into FGL-PA solu-tion to reduce the PH value and accordingly induce self-assembly.The morphological features of the assembled material were studied by transmission electron microscope.NSCs were cultured and added with self-assembled FGL-PA.CCK-8 kit was used to test its effect on the proliferation of NSCs.The differentiation of NSCs was also tested after FGL-PA assembled material added.The experimental results showed that FGL-PA could be self-assembled to form a hydrogel.TEM analysis showed the self-assembled hydrogel was nanofibers with diameter of 10-20 nm and length of hundreds nanometers.FGL-PA with concentrations of 50,100,or 200 mg/L could promote the proliferation of NSCs,and absorbance of them was increased(P＜0.05).The rate of neurons differentiated from NSCs was im-proved greatly by FGL-PA assembled material compared with control(P＜0.05).The findings suggested that FGL-PA could self-assemble to nanofiber hydrogel,which had good biocompatibility with NSCs.
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