利用兰州重离子研究装置(HIRFL)提供的12C6+离子束辐照菘蓝干种子(辐照剂量为10，35，60，90和140 Gy，剂量率20 Gy/min)，探讨了重离子束辐照对菘蓝M1代的生物学效应。研究发现，不同剂量的12C6+离子束辐照后，菘蓝种子的发芽率、成苗率、株高、根长和根冠比等生物学性状以及对菘蓝中靛玉红和4(3H)喹唑酮含量均发生了变化，其中株高和根长随辐照剂量的增加而降低；菘蓝叶和根中的4(3H)喹唑酮和靛玉红的含量随辐照剂量增加呈马鞍形增加关系。这表明：12C6+重离子束辐照菘蓝种子具有明显的当代损伤效应,并可显著提高菘蓝中靛玉红和4(3H)喹唑酮的含量，其辐照适宜诱变剂量为35 Gy。
To investigate the M1 biological effects of heavy ions on Isatis indigotica Fort, its dry seeds were irradiated by 12C6+ beam with the dose of 0, 10, 35, 60, 90 and 140 Gy respectively,at the rate of 20 Gy/min delivered by the Heavy Ion Research Facility in Lanzhou (HIRFL). The results showed that biological characters such as germinating rate, germinating potential, survival rate, plant height, root height and root-shoot ratio were changed after irradiation. Moreover, the plant height and root height decreased in a dos dependent manner. The indirubin and 4(3H) quinazolinone content of Isatis indigotica Fort was improved and exhibited obviously“saddle”trends with irradiation dose increasing. Data suggest that exposure with low-dose 12C6+ to seeds of Isatis indigotica Fort has obvious injury effects at the first generation, and the active ingredient content of Isatis indigotica Fort may be improved by carbon ion beam irradiation. It is concluded that the suitable irradiation dose of mutation breeding is 35 Gy for the seeds of Isatis indigotica Fort.
参考文献
| [1] | DANZ H, STOYANOVA S, WIPPICH P, et al. Planta Medica, 2001, 67(5):411. |
| [2] | LIANG Jianping, LI Xuehu, LU Xihong, et al. Nuclear Physics Reviews, 2010, 27(3):284.(in Chinese)(梁剑平, 李雪虎, 陆锡宏, 等. 原子核物理评论, 2010, 27(3):284.) |
| [3] | WU Zhenhua, ZHANG Hong, WANG Xinyu, et al. Nuclear Physi-cs Reviews, 2010, 27(3):335.(in Chinese)(武振华,张红,王新宇,等.原子核物理评论, 2010, 27(3):335.) |
| [4] | YU Zengliang. On Ion Beam Applied to Bio-tech[M]. Hefei：An-hui Sci TechPress, 1998, 195-222.(in Chinese)(余增亮.离子束生物技术引论[M].合肥：安徽科学技术出版社, 1998, 195-222.) |
| [5] | DONG Xichun, LI Wenjian, HE Jinyu, et al. Nuclear Techniques, 2009, 32(2):146.(in Chinese)(董喜存,李文建,何金玉,等.核技术, 2009, 32(2):146.) |
| [6] | JIA Yanyan, DENG Chuanliang, GAO Jun, et al. Acta Agriculturae Nucleatae Sinica, 2011, 25(5):904.(in Chinese)(贾彦彦,邓传良,高俊,等.核农学报, 2011, 25(5):904.) |
| [7] | ZHOU Libing, LI Wenjian, QU Ying, et al. Nuclear Physics Re-views, 2008, 23(2):165.(in Chinese)(周利斌,李文建,曲颖,等.原子核物理评论, 2008, 23(2):165.) |
| [8] | FENG Wenxin, ZHANG Shumei. Journal of Shanxi Agricultural University, 1997, 17(1):21.(in Chinese)(冯文新,张素梅.山西农业大学学报, 1997, 17(1):21.) |
| [9] | CHEN Yiping, WANG Xunling, HAN Jing. Chinese Herbal Medicines, 2003, 34(11):1054.(in Chinese)(陈怡平,王勋陵,韩静.中草药, 2003, 34(11):1054.) |
| [10] | CHEN Yiping, WANG Xunling. Laser Technology, 2004, 28(1):42.(in Chinese)(陈怡平,王勋陵.激光技术, 2004, 28(1):42.) |
| [11] | YAMAGUCHI H, NAGATOMI S, MORISHITA T. Nucl Instr and Meth B, 2003, 206:561. |
| [12] | YAMAGUCHI H, MORISHITA T, DEGI K, et al. Nippon Gen-shiryoku Kenkyujo JAERI. 2002, 1:36. |
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