首先采用葡萄糖与精氨酸水热合成碳量子点,并研究了碳量子点的结构和光谱特性.然后使用激光微量热泳动技术(MST)研究碳量子点与4种三磷酸脱氧核苷酸分子(dNTP)和单双链DNA间的亲和行为.实验结果表明制备的碳量子点粒径均一、具有良好的荧光性等,碳量子点与dNTP和MST-1/2ssDNA间存在着亲和行为,而碳量子点与dsDNA间的亲和力不明显;同时发现碳量子点与dNTP和ssDNA分子间通过氢键形式结合,其结合强弱与dNTP分子结构及ssDNA碱基序列排布有关,而dsDNA分子结构稳定,没有多余氨基位点,与碳量子点无明显结合.
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