利用透明质酸钠(Sodium hyaluronate,简称SH)和魔芋葡甘聚糖(Konjac glucomannan,简称KGM)制备共混膜SH/KGM,通过膜的干预减少或阻止硬膜外瘢痕的形成.选择人皮肤成纤维细胞为种子细胞,采用流式细胞术和半定量PCR方法检测细胞的纯度;通过体外移植的方法将成纤维细胞移植到SH/KGM共混膜表面,细胞形态学观察发现细胞由原来的梭形变成不规则的圆形,且细胞能够粘附于SH/KGM共混膜的表面,但移植细胞在移植后的2天、5天和7天内均没有观察到增殖.这种现象表明共混膜SH/KGM具有防止硬膜外瘢痕粘连的作用,可以作为腰椎手术的介入材料.
参考文献
| [1] | Barron L;Wynn TA .Fibrosis is regulated by Th2 and Th17 responses and by dynamic interactions between fibroblasts and macrophages.[J].American Journal of Physiology,2011(5Part 1):G723-728. |
| [2] | Ma L;Gan C;Huang Y et al.Comparative proteomic analysis of extracellular matrix proteins secreted by hypertrophic scar with normal skin fibroblasts[J].Bums Trauma,2014,2(02):76. |
| [3] | Parapuram SK;Shi wen X;Elliott C;Welch ID;Jones H;Baron M;Denton CP;Abraham DJ;Leask A .Loss of PTEN expression by dermal fibroblasts causes skin fibrosis.[J].The Journal of Investigative Dermatology,2011(10):1996-2003. |
| [4] | Key J A;Ford L T .Experimental intervertebral-disc lesions[J].J Bone Joint Surgery(American),1948,30(03):621. |
| [5] | Chen, G.;Gulbranson, D.R.;Hou, Z.;Bolin, J.M.;Ruotti, V.;Probasco, M.D.;Smuga-Otto, K.;Howden, S.E.;Diol, N.R.;Propson, N.E.;Wagner, R.;Lee, G.O.;Antosiewicz-Bourget, J.;Teng, J.M.C.;Thomson, J.A. .Chemically defined conditions for human iPSC derivation and culture[J].Nature methods,2011(5):424-429. |
| [6] | Trond Aasen;Juan Carlos Izpisua Belmonte .Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells[J].Nature protocols erecipes for researchers,2010(2):371-382. |
| [7] | Zhu Xiangqing;Pan Xinghua;Hu Jifan .Transient in vitro epigenetic reprogramming of skin fibroblasts into multipotent cells[J].BIOMATERIALS,2010,31:2779. |
| [8] | Amit M.;Margulets V.;Segev H.;Shariki K.;Laevsky I.;Coleman R. Itskovitz-Eldor J. .Human feeder layers for human embryonic stem cells[J].Biology of Reproduction,2003(6):2150-2156. |
| [9] | Ejiri H;Nomura T;Hasegawa M.Use of synthetic serum-free medium for culture of human dermal fibroblasts to establish an experimental system similar to living dermis[J].CYTOTECHNOLOGY |
| [10] | Mah W;Jiang G;Olver D et al.Human gingival fibroblasts display a non-fibrotic phenotype distinct from skin fibroblasts in three-dimensional cultures[J].Plos One,2014,9(03):e90715. |
| [11] | 李海红,周岗,付小兵,屈振亮,孙同柱,顾绍峰.人皮肤成纤维细胞的培养和鉴定[J].中国危重病急救医学,2005(02):89-91. |
| [12] | 蒋知新,张清华,艾民,沙杭,高毅,卢海.人皮肤成纤维细胞来源诱导性多潜能干细胞的建立和鉴定[J].中国医师杂志,2011(08):1026-1029,1032. |
| [13] | 曹晨华,纪丽曼,耿娅,刘国芳.人皮肤成纤维细胞系的鉴定及老化检测[J].河北医药,2009(16):2040-2042. |
| [14] | 鲍荣辉,刘先哲,周军.人成纤维细胞的原代培养及鉴定[J].新乡医学院学报,2011(05):577-579. |
| [15] | Zhu, Xiang-Qing;Ruan, Guang-Ping;Chen, Qing-Hua;Pang, Rong-Qing;Cai, Xue-Ming;Li, Zi-An;Chen, Cui-Zhu;Lei, Li;Wang, Qiang;He, Jie;Zhao, Jing;Pan, Xing-Hua .Transient Epigenetic Reprogramming Human Skin Fibroblasts Differentiates into Cells with Multipotentiality In Vitro and In Vivo[J].Cytologia : International journal of cytology,2012(2):251-259. |
| [16] | Burns J W;Calt M J;Burgess L S et al.Preclinical evaluation of seprafilm bioresorbable membrane[J].European Journal of surgery,1977,577(S):40. |
| [17] | Foti C;Cisari C;Carda S et al.A prospective observational study of the clinical efficacy and safety of intra-articular sodium hyaluronate in synovial joints with osteoarthritis[J].Eur J Phys Rehabil Med,2011,47(03):407. |
| [18] | Sylvain Roy;Huyen Do Thi;Michael Feusier;André Mermoud .Crosslinked sodium hyaluronate implant in deep sclerectomy for the surgical treatment of glaucoma.[J].European journal of ophthalmology,2012(1):70-76. |
| [19] | Stawicki S P;Green J M;Martin N D et al.Results of a prospective,randomized,controlled study of the use of carboxymethylcellulose sodium hyaluronate adhesion barrier in trauma open abdomens[J].SURGERY,2014,156(02):419. |
| [20] | de Paula Pereira Junior A;Fasolin R P;Sossa F A C et al.Results evaluation of the use of intra-articular sodium hyaluronate in the post-operative knee arthroscopy[J].Revista Brasileira de Ortopedia(English Edition),2014,49(01):37. |
| [21] | 王益民,韦福康,刘敏.成纤维细胞与创伤修复的研究进展[J].中国修复重建外科杂志,2000(02):126-128. |
| [22] | 石慧娟.成纤维细胞及其相关因素在无瘢痕愈合中的研究进展[J].四川解剖学杂志,2011(01):45-47. |
| [23] | Zhang P;Li J;Tang X et al.Dracorhodin perchlorate induces apoptosis in primary fibroblasts from human skin hypertrophic scars via participation of caspase-3[J].Europen Journal of Pharmacology,2014,728:82. |
| [24] | Arno,A.I.;Gauglitz,G.G.;Barret,J.P.;Jeschke,M.G. .New molecular medicine-based scar management strategies[J].Burns: Including Thermal Injury,2014(4):539-551. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%