采用静电纺制备纳米纤维膜,接着通过高温碳化技术处理得到碳纳米纤维膜(ECNFs),再通过共混得到漆酶-全氟磺酸聚四氟乙烯共聚物-纳米碳纤维玻碳电极(laccase-Nafion-ECNFs/GCE)并制备新酚醛传感器.采用拉曼荧光显微分光计和Nicolet iS10傅里叶红外光谱仪对ECNFs进行分析;用循环伏安法对laccase-Nafion/GCE、laccase/GCE、laccase-Nafion-ECNFs/GCE进行了检测并测试扫描速率对laccase-Nafion-ECNFs/GCE效果的影响;采用方波伏安法对laccase-Nafion/GCE、laccase/GCE、laccase-Nafion-ECNFs/GCE进行了检测并测试对苯二酚浓度对laccase-Nafion-ECNFs/GCE效果的影响.结果表明:实验获得了ECNFs,且ECNFs的表面存在大量的羧基基团,有利于提高ECNFs的电催化性能和生物相容性;ECNFs的添加,提高了复合材料的导电率并增加了玻碳电极表面到漆酶中心的电子转移速率;漆酶在laccase-Nafion-ECNFs复合材料和玻碳电极表面很容易发生电子传输;ECNFs的修饰强烈地增加了玻碳电极对对苯二酚的电催化作用;该生物传感器的检测限为0.32 μmol/L,线性范围为1～20μmol/L,可满足环境监测的要求.这种基于电纺纳米碳纤维的新型生物传感器装置具有很大的应用潜力.
参考文献
| [1] | Ryoo R.;Kruk M.;Jaroniec M.;Joo SH. .Ordered mesoporous carbons[J].Advanced Materials,2001(9):677-681. |
| [2] | Kim T W;Park I S;Ryoo R .A synthetic route to ordered mesoporous carbon materials with graphitic pore walls[J].ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2003,42(36):4375. |
| [3] | Zhonglai Li;Jing Zhang;Ying Li;Yejun Guan;Zhaochi Feng .Preparation and characterization of ordered mesoporous carbons on SBA-15 template[J].Journal of Materials Chemistry: An Interdisciplinary Journal dealing with Synthesis, Structures, Properties and Applications of Materials, Particulary Those Associated with Advanced Technology,2006(14):1350-1354. |
| [4] | Joo S H;Choi S J;Oh I et al.Ordered nanoporous arrays of carbon supporting high dispersions of platinum nanoparticles[J].NATURE,2001,412(6843):169. |
| [5] | Zhou HS;Zhu SM;Honma I;Seki K .Methane gas storage in self-ordered mesoporous carbon (CMK-3)[J].Chemical Physics Letters,2004(4-6):252-255. |
| [6] | X.Y. Tao;X.B. Zhang;L. Zhang .Synthesis of multi-branched porous carbon nanofibers and their application in electrochemical double-layer capacitors[J].Carbon: An International Journal Sponsored by the American Carbon Society,2006(8):1425-1428. |
| [7] | Yang K S;Kim C;Park S H et al.Fabrications and electrochemical properties of activated carbon nanofibers from electrospinning of various polymers[J].J Biomed Nanotechn,2006,2(02):103. |
| [8] | Kim C .Electrochemical characterization of electrospun activated carbon nanofibres as an electrode in supercapacitors[J].Journal of Power Sources,2005,142(1-2):382. |
| [9] | Cesar Merino .Carbon nanofibres and activated carbon nanofibres as electrodes in supercapacitors[J].Carbon: An International Journal Sponsored by the American Carbon Society,2005(3):551-557. |
| [10] | De Jong KP.;Geus JW. .Carbon nanofibers: Catalytic synthesis and applications [Review][J].Catalysis Reviews. Science and Engineering,2000(4):481-510. |
| [11] | 赵雪飞,邱介山,孙业新,郝策,孙天军,崔凌威.电弧放电法制备煤基炭纳米纤维及开口竹节状碳纳米管[J].新型炭材料,2009(02):109-113. |
| [12] | Guo T;Nikolaev P;Rinzler A G et al.Self-assembly of tubular fullerenes[J].Journal of Physical Chemistry,1995,99(27):10694. |
| [13] | Boskovic B O et al.Large-area synthesis of carbon nanofibres at room temperature[J].NATURE MATERIALS,2002,1(03):165. |
| [14] | Yu Wang;Santiago Serrano;Jorge J. Santiago-Aviles .Raman characterization of carbon nanofibers prepared using electrospinning[J].Synthetic Metals,2003(3):423-427. |
| [15] | Liu Y;Hou HQ;You TY .Synthesis of carbon nanofibers for mediatorless sensitive detection of NADH[J].Electroanalysis,2008(15):1708-1713. |
| [16] | Huang JS;Liu Y;Hou HQ;You TY .Simultaneous electrochemical determination of dopamine, uric acid and ascorbic acid using palladium nanoparticle-loaded carbon nanofibers modified electrode[J].Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices,2008(4):632-637. |
| [17] | Liu, Y;Teng, H;Hou, HQ;You, TY .Nonenzymatic glucose sensor based on renewable electrospun Ni nanoparticle-loaded carbon nanofiber paste electrode[J].Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices,2009(11):3329-3334. |
| [18] | Singh, S.;Jain, D.V.S.;Singla, M.L..Sol-gel based composite of gold nanoparticles as matix for tyrosinase for amperometric catechol biosensor[J].Sensors and Actuators, B. Chemical,2013:161-169. |
| [19] | Chawla, Sheetal;Rawal, Rachna;Pundir, C. S. . .Fabrication of polyphenol biosensor based on laccase immobilized on copper nanoparticles/chitosan/multiwalled carbon nanotubes/polyaniline-modified gold electrode[J].Journal of Biotechnology,2011(1):39-45. |
| [20] | Minussi RC;Pastore GM;Duran N .Potential applications of laccase in the food industry [Review][J].Trends in food science & technology,2002(6/7):205-216. |
| [21] | He Q P;Joy D C;Keffer D J .Nanoparticle adhesion in proton exchange membrane fuel cell electrodes[J].Journal of Power Sources,2013,241:634. |
| [22] | Kumar S;Vicente-Beckett V .Glassy carbon electrodes modified with multiwalled carbon nanotubes for the determination of ascorbic acid by square-wave voltammetry[J].Beilstein J Nanotechn,2012,3:388. |
| [23] | Sakae Takenaka;Yo Tomikubo;Emi Kato;Kiyoshi Otsuka .Sequential production of H_2 and CO over supported Ni catalysts[J].Fuel,2004(1):47-57. |
| [24] | Rand, E.;Periyakaruppan, A.;Tanaka, Z.;Zhang, D.A.;Marsh, M.P.;Andrews, R.J.;Lee, K.H.;Chen, B.;Meyyappan, M.;Koehne, J.E..A carbon nanofiber based biosensor for simultaneous detection of dopamine and serotonin in the presence of ascorbic acid[J].Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices,2013:434-438. |
| [25] | S. Kumar;B. Lively;L.L. Sun .Highly dispersed and electrically conductive polycarbonate/ oxidized carbon nanofiber composites for electrostatic dissipation applications[J].Carbon: An International Journal Sponsored by the American Carbon Society,2010(13):3846-3857. |
| [26] | Ratinac, K.R.;Yang, W.;Gooding, J.J.;Thordarson, P.;Braet, F. .Graphene and related materials in electrochemical sensing[J].Electroanalysis,2011(4):803-826. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%