研究不同浓度Mg-6Zn合金浸提液对大鼠肠上皮细胞IEC-6的凋亡及凋亡相关基因Caspase-3表达的影响.将Mg-6Zn合金制成不同浓度(20％和40％)的浸提液体外培养大鼠肠上皮细胞IEC-6,并以含10％胎牛血清的高糖DMEM(Dulbecco's modified Eagle's medium)培养基作为阴性对照.采用噻唑蓝(MTT)法通过计算细胞相对增殖率检测不同浓度合金浸提液对IEC-6细胞的毒性作用;采用流式细胞仪检测不同浓度合金浸提液对IEC-6细胞凋亡的影响;采用逆转录聚合酶链反应(RT-PCR)法检测不同浓度合金浸提液对IEC-6细胞凋亡相关基因Caspase-3 mRNA表达的影响.实验结果表明,20％和40％两种浓度的镁合金浸提液在体外培养IEC-6细胞第七天时细胞毒性分级分别为0级和1级,属于无毒性,符合医用材料细胞毒性分级的合格标准.40％和20％浓度镁合金浸提液中,IEC-6细胞的凋亡比例及Caspase-3活化度相对于对照组都增高,差异有统计学意义(P＜0.05),40％浓度组中,IEC-6细胞的凋亡比例及Caspase-3活化度相对于20％浓度组增高,差异有统计学意义(P＜0.05),说明Mg-6Zn合金浸提液在一定范围内处于较高浓度时易诱导IEC-6细胞的凋亡及Caspase-3的表达,且随浓度升高影响越大.
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