一种简易同时蒸馏萃取方法用于八角茴香挥发油提取及其GC-MS分析

应用化学, 2015, 32(3): 356-361. doi: 10.11944/j.issn.1000-0518.2015.03.140196

一种简易同时蒸馏萃取方法用于八角茴香挥发油提取及其GC-MS分析

孙玉琼 ^1, , 夏东海 ^2, , 董新荣 ^3, , 李霞 ^4,

1.湖南农业大学理学院长沙410128

2.湖南农业大学理学院长沙410128

3.湖南农业大学理学院长沙410128

4.湖南农业大学理学院长沙410128

基金项目: Hunan Science and Technology Bureau Foundation (No.2011FJ6054)湖南省科技厅基金项目(2011FJ6054)

关键词：同时蒸馏萃取 , 水蒸汽蒸馏 , 气相色谱/质谱 , 挥发油 , 八角茴香

A Simple Simultaneous Distillation/Solvent Extraction Method Coupled with Gas Chromatography-Mass Spectrometry for the Analysis of Volatile Oils of Illicium Verum

Keywords： simultaneous distillation extraction , hydrodistillation , gas chromatography-mass spectrometry , volatile oil , Illicium Verum

设计了一种简易同时蒸馏萃取(SSDE)装置并用于八角茴香挥发油提取及其气相色谱-质谱法(GC-MS)分析.该装置由圆底烧瓶、恒压滴液漏斗和回流冷凝管构成.通过对八角茴香挥发油组分的GC-MS分析,比较SSDE法和水蒸汽蒸馏提取法(HD)提取植物源挥发油的效果.结果表明,SSDE法所得挥发油相对含量在0.1％以上的19种化合物占挥发油总量的98.84％(相对标准偏差(RSD)(n=3)均不大于5.59％,其中RSD＜5％的17种化合物占鉴定组分89.5％.SSDE法和HD法挥发油平均收率分别为7.30％(RSD=2.37％(n=5))和6.67％ (RSD =5.26％ (n =5)).SSDE法简便、快速,适用于八角茴香挥发油的提取.

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