设计合成了一种新型含磷的水溶性三足体衍生物[L:N-二(吡啶-二氨基乙酰基)甲基磷酸]及其Eu(Ⅲ)的配合物,用红外吸收光谱、元素分析、差热-热重和紫外光谱法等技术手段对该配合物进行了表征,用荧光光谱法研究了室温下该配合物和牛血清白蛋白(BSA)的相互作用.结果表明,配体与苦味酸铕形成1∶1型配合物Eu(pic)3L;配合物与BSA之间有很强的结合作用;配合物对BSA内源荧光的猝灭方式为静态猝灭;配合物与BSA的作用力为分子间氢键和范德华力.分别考察了Fe3+和Cu2对配合物与BSA结合作用的影响,证明Fe3+和Cu2能够以金属离子桥键与配合物结合使配合物-BSA的稳定性增强.根据Foster型偶极-偶极无辐射能量转移机理可知,配合物可以和BSA以偶极-偶极无辐射进行能量传递.
参考文献
| [1] | 吴明旭;赵治;周晶;王玉芹.有机磷农药对枯草芽胞杆菌生长和生理特征的影响[J].畜牧与饲料科学,2013(3):10-13. |
| [2] | Vitha, T;Kubicek, V;Kotek, J;Hermann, P;Elst, LV;Muller, RN;Lukes, I;Peters, JA.Gd(III) complex of a monophosphinate-bis(phosphonate) DOTA analogue with a high relaxivity; Lanthanide(III) complexes for imaging and radiotherapy of calcified tissues[J].Dalton transactions: An international journal of inorganic chemistry,200917(17):3204-3214. |
| [3] | Hui Xu;Lian-Hui Wang;Xu-Hui Zhu;Kun Yin;Gao-Yu Zhong.Application of Chelate Phosphine Oxide Ligand in Eu~(III) Complex with Mezzo Triplet Energy Level,Highly Efficient Photoluminescent,and Electroluminescent Performances[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,20067(7):3023-3029. |
| [4] | Garry E. Kiefer;Mark Woods.Solid State and Solution Dynamics of Pyridine Based Tetraaza-Macrocyclic Lanthanide Chelates Possessing Phosphonate Ligating Functionality (Ln-PCTMB): Effect on Relaxometry and Optical Properties[J].Inorganic Chemistry: A Research Journal that Includes Bioinorganic, Catalytic, Organometallic, Solid-State, and Synthetic Chemistry and Reaction Dynamics,200924(24):11767-11778. |
| [5] | Xu, X.-W.;Li, X.-J.;Zhan, S.-H.;Li, Y.-T.;Wu, Z.-Y.;Yan, C.-W..Synthesis and structure of a new tetracopper(II) complex bridged both by oxamido and phenolato groups: Cytotoxic activity, and reactivity towards DNA and BSA[J].Journal of Molecular Structure,2013:28-36. |
| [6] | 霍丽娜;刘伟;杨天林;李晓红.新型铈苦味酸配合物的合成、表征及其与白蛋白相互作用[J].稀土,2014(2):37-43. |
| [7] | 赵龙;卢继新;项振玲;李娟;徐靖源.多柔比星稀土配合物与人血清白蛋白相互作用的研究[J].分析科学学报,2011(5):581-585. |
| [8] | 马学兵;傅相锴;王强;王宁.含羟基、膦酸根水溶性膦配体的合成[J].应用化学,2002(12):1154-1157. |
| [9] | 高继红;杨天林;吴根亮.2,2,2-胺三乙酰-苯胺及其稀土配合物的合成、表征与荧光性质[J].稀有金属快报,2007(7):29-33. |
| [10] | 谢远武.摩尔比法测定配合物组成和稳定常数的理论探讨[J].化学通报,1988(2):39-42. |
| [11] | Wei Liu;Xiao-Hong Li;Li-Na Huo.Synthesis, Luminescence and DNA-Binding Properties of Eu(III) and Tb(III) Complexes with Amide-Based 2,3-Dihydroxynaphthalene Derivative[J].Asian Journal of Chemistry: An International Quarterly Research Journal of Chemistry,201318(18):10465-10469. |
| [12] | Vicente Sanchez C;Egido J;Sanchez Gonzalez PD.Effect of the flavonoid quercetin on cadmium-induced hepatotoxicity.[J].Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research,20086(6):2279-2287. |
| [13] | 杨美玲;杨培菊;宋玉民.芦丁金属配合物的合成、表征及与血清白蛋白的相互作用[J].无机化学学报,2005(4):483-489. |
| [14] | 田伦富;刘忠芳;胡小莉;孔玲;刘绍璞.[Hg（SCN）4]2-对蛋白质的荧光猝灭反应及其分析应用[J].高等学校化学学报,2012(1):59-65. |
| [15] | 林海彬;郑琳;林玉琴;周朝晖.邻菲啰啉和氨三乙酸钴配合物与牛血清白蛋白相互作用的荧光分析[J].高等学校化学学报,2013(8):1818-1825. |
| [16] | 杨曼曼;席小莉;杨频.用荧光猝灭和荧光加强两种理论研究喹诺酮类新药与白蛋白的作用[J].高等学校化学学报,2006(4):687-691. |
| [17] | 黄英;王娟;郭改英;陶朱;薛赛凤;祝黔江;周清娣.光谱法研究硫鸟嘌呤与七元瓜环及牛血清白蛋白的超分子相互作用[J].高等学校化学学报,2013(2):375-380. |
| [18] | Peng, L.;Wei, R.;Li, K.;Zhou, Z.;Song, P.;Tong, A..A ratiometric fluorescent probe for hydrophobic proteins in aqueous solution based on aggregation-induced emission[J].The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry,20137(7):2068-2072. |
| [19] | 孙启龙;王朝阳;任碧野;刘新星;曾钫;刘平;童真.添加盐对磺酸基聚电解质分子内及分子间荧光非辐射能量转移的影响[J].高等学校化学学报,2003(9):1695-1699. |
| [20] | Guo XQ.;Li L.;Lakowicz JR.;Castellano FN..Use of a long lifetime Re(I) complex in fluorescence polarization immunoassays of high-molecular weight analytes[J].Analytical chemistry,19983(3):632-637. |
| [21] | 孙长峰;郭娜.微量元素铁对人体健康的影响[J].微量元素与健康研究,2011(2):64-66. |
| [22] | 尚永辉;李华;孙家娟;郑敏燕.核黄素与牛血清白蛋白相互作用的荧光光谱研究[J].分析科学学报,2010(1):67-70. |
| [23] | 肖建波 .多酚类化合物与血清白蛋白相互作用的结构-结合力关系、理论模型和应用研究[D].中南大学,2009. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%