以对羟基苯甲酸(p-HBA)为模板分子,丙烯酰胺(AM)为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合法在乙腈溶剂中制备了p-HBA印迹聚合物微粒,研究了p-HBA加入量及聚合反应体系的总浓度对印迹聚合物结合性能的影响,采用色谱法对其进行了评价.结果表明,p-HBA的加入量及反应体系的总浓度对结合性能均有影响,当p-HBA加入量为1.0 mmol(与AM物质的量比为1:2)时,在37.5 mL乙腈中制得印迹聚合物P2对p-HBA具有高的亲和力(k'=4.01)和选择性.将印迹聚合物P2作为固相萃取填料,研究了分子印迹固相萃取p-HBA的方法,测得2种不同载样模式下p-HBA的柱容量分别为6.91 μg/100 mg和1.93 μg/100 mg,测得天麻样品中p-HBA的加标回收率为76.8％ ～86.6％(RSD =3.4％ ～6.2％)(n=3).结果表明,采用沉淀聚合法以AM为功能单体制备的p-HBA印迹聚合物微粒适宜作为固相萃取填料,可实现天麻样品中p-HBA的选择性分离净化.
参考文献
| [1] | Cheong, W.J.;Ali, F.;Choi, J.H.;Lee, J.O.;Yune Sung, K..Recent applications of molecular imprinted polymers for enantio-selective recognition[J].Talanta: The International Journal of Pure and Applied Analytical Chemistry,2013:45-59. |
| [2] | Díaz-Díaz, G.;Antu?a-Jiménez, D.;Carmen Blanco-López, M.;Jesús Lobo-Casta?ón, M.;Miranda-Ordieres, A.J.;Tu?ón-Blanco, P..New materials for analytical biomimetic assays based on affinity and catalytic receptors prepared by molecular imprinting[J].TrAC: Trends in Analytical Chemistry,2012:68-80. |
| [3] | Mostafa Khajeh;Esmael Sanchooli.Imprinted polymer particles for preconcentration of copper from water and biological samples[J].Environmental chemistry letters,20112(2):177-183. |
| [4] | 胡艳云;徐慧群;姚剑;吕亚宁;宋伟;李文静;韩芳;郑平.分子印迹固相萃取-液相色谱-质谱法测定果蔬中20种三唑类农药残留[J].分析化学,2014(2):227-232. |
| [5] | 薛敏;王安;王瑜;王丹;吕稚;孟子晖;张维冰.分子印迹固相萃取技术检测江水、尿液及牛奶中雌激素残留[J].分析化学,2011(6):793-798. |
| [6] | 蒋旭红;涂伟萍.分子印迹聚合物微球制备方法研究进展[J].材料导报,2007(12):52-55. |
| [7] | 雷秀兰;易翔;袁洋;曾延波;李蕾.罗丹明B分子印迹聚合物微球的合成及其在固相萃取中的应用[J].应用化学,2011(5):531-536. |
| [8] | 许苗苗;王素素;李辉;黄宇琳;逯翠梅;蒋剑波.沉淀聚合法制备葛根素印迹微球及其固相萃取葛根粉[J].食品科学,2015(10):11-15. |
| [9] | 杜自卫;刘学涌;赵小东.TNT分子印迹聚合物微球的合成与性能研究[J].高分子学报,2010(3):360-365. |
| [10] | Chen, F.-F.;Wang, G.-Y.;Shi, Y.-P..Molecularly imprinted polymer microspheres for solid-phase extraction of protocatechuic acid in Rhizoma homalomenae[J].Journal of separation science,201119(19):2602-2610. |
| [11] | S.J.Chem.我国对羟基苯甲酸的现状和发展趋势[J].精细与专用化学品,2003(12):6-7. |
| [12] | 王铁杰;李璐;江坤;殷果;叶文才;张晓琦.RP-HPLC法同时测定榕树须中原儿茶酸、对羟基苯甲酸和原儿茶醛的含量[J].沈阳药科大学学报,2014(9):706-710,725. |
| [13] | 闫宝庆;张晖芬;逄楠楠;陈晓辉;于治国;毕开顺.RP-HPLC同时测定天麻中对羟基苯甲酸和4,4'-二羟基二苄砜的含量[J].药物分析杂志,2010(9):1729-1731. |
| [14] | Tieli Zhang;Feng Liu;Wen Chen;Jun Wang;Kean Li.Influence of intramolecular hydrogen bond of templates on molecular recognition of molecularly imprinted polymers[J].Analytica chimica acta,20011/2(1/2):53-61. |
| [15] | 孙宝维;武利庆;李元宗.由不同功能单体合成的对羟基苯甲酸分子印迹聚合物识别特性的实验和理论研究[J].化学学报,2004(6):598-602. |
| [16] | 王车礼;赵兴丽;方磊;张琪;钟璟;李亮.对羟基苯甲酸分子印迹聚合物合成功能单体选择与分子识别性能[J].化工学报,2011(7):1938-1943. |
| [17] | Shuyun Shi;Junfang Guo;Qingping You.Selective and simultaneous extraction and determination of hydroxybenzoic acids in aqueous solution by magnetic molecularly imprinted polymers[J].Chemical engineering journal,2014:485-493. |
| [18] | 张铁莉;刘锋;李克安.模板分子中作用基团的数目及位置对印迹聚合物印迹效应的影响[J].高等学校化学学报,2010(6):1126-1130. |
| [19] | Nathaporn Phutthawong;Mookda Pattarawarapan.Synthesis of Highly Selective Spherical Caffeine Imprinted Polymers via Ultrasound-Assisted Precipitation Polymerization[J].Journal of Applied Polymer Science,20136(6):3893-3899. |
| [20] | Chunyan Zhao;Jiangdong Dai;Zhiping Zhou;Xiaohui Dai;Yongli Zou;Ping Yu;Tianbian Zou;Chunxiang Li;Yongsheng Yan.One-Pot Method for Obtaining Hydrophilic Tetracycline-Imprinted Particles via Precipitation Polymerization in Ethanol[J].Journal of Applied Polymer Science,20147(7):40071-1-40071-11. |
| [21] | Gonzalez-Marino, I;Quintana, JB;Rodriguez, I;Rodil, R;Gonzalez-Penas, J;Cela, R.Comparison of molecularly imprinted, mixed-mode and hydrophilic balance sorbents performance in the solid-phase extraction of amphetamine drugs from wastewater samples for liquid chromatography-tandem mass spectrometry determination[J].Journal of chromatography, A: Including electrophoresis and other separation methods,200948(48):8435-8441. |
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