选择芦笋(Asparagus Officinalis L)新鲜茎块和两种干燥茎块为代表性植物组织样品,建立了一种直接检测芦笋中多种化学成分的内部萃取电喷雾电离质谱(iEESI-MS)分析方法.在正离子检测模式下,选择甲醇(CH3OH)作萃取溶剂,在无需样品预处理的条件下对芦笋组织样品进行直接质谱分析,获得了组织样品在m/z 50～ 1000范围内的化学指纹谱图,并通过目标离子的碰撞诱导解离(CID)实验,鉴定了对药物和自然产品研发具有重要意义的糖类、氨基酸、生物碱以及芦丁等多种代表性的营养成分.本方法具有无需样品预处理、样品耗量少、操作简便、分析速度快(单个样品的分析时间少于1min)等优点,为食源性植物组织样品的快速分析提供了一种质谱分析新方法.
参考文献
| [1] | 李翠霞;毛箬青;李志忠;王永刚;刘晓风.芦笋营养成分的分析评价[J].现代食品科技,2011(10):1260-1263. |
| [2] | 孙春艳;赵伯涛;郁志芳;钱骅;黄晓德.芦笋的化学成分及药理作用研究进展[J].中国野生植物资源,2004(5):1-5. |
| [3] | 康旭;袁江兰;邓川;孟鸳.绿芦笋茶挥发性风味成分的GC-MS分析[J].农产品加工·学刊,2010(12):56-58. |
| [4] | Kim, B. Y.;Cui, Z. G.;Lee, S. R.;Kim, S. J.;Kang, H. K.;Lee, Y. K.;Park, D. B..Effects of <i>Asparagus officinalis</i> extracts on liver cell toxicity and ethanol metabolism.[J].Journal of Food Science,20097(7):H204-H208. |
| [5] | Wang M;Tadmor Y;Wu QL;Chin CK;Garrison SA;Simon JE.Quantification of Protodioscin and Rutin in Asparagus Shoots by LC/MS and HPLC Methods.[J].Journal of Agricultural and Food Chemistry,200321(21):6132-6136. |
| [6] | Zhong, Chunge;Jiang, Chunyu;Xia, Xichun;Mu, Teng;Wei, Lige;Lou, Yuntian;Zhang, Xiaoshu;Zhao, Yuqing;Bi, Xiuli.Antihepatic Fibrosis Effect of Active Components Isolated from Green Asparagus (Asparagus officinalis L.) Involves the Inactivation of Hepatic Stellate Cells[J].Journal of Agricultural and Food Chemistry,201526(26):6027-6034. |
| [7] | Hafizur, R. M;Kabir, N;Chishti, S..Asparagus officinalis extract controls blood glucose by improving insulin secretion and beta-cell function in streptozotocin-induced type 2 diabetic rats.[J].The British Journal of Nutrition,20129(9):1586-1595. |
| [8] | Chua,L.S..A review on plant-based rutin extraction methods and its pharmacological activities[J].Journal of Ethnopharmacology: An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs,20133(3):805-817. |
| [9] | 陈焕文;胡斌;张燮.复杂样品质谱分析技术的原理与应用[J].分析化学,2010(8):1069-1088. |
| [10] | Tiqiang Zhang;Wei Zhou;Wei Jin;Jianguang Zhou;Eric Handberg;Zhiqiang Zhu;Huanwen Chen;Qinhan Jin.Direct desorption/ionization of analytes by microwave plasma torch for ambient mass spectrometric analysis[J].Journal of mass spectrometry: JMS,20136(6):669-676. |
| [11] | Joshua S. Wiley;Jacob T. Shelley;R. Graham Cooks.Handheld Low-Temperature Plasma Probe for Portable "Point-and-Shoot" Ambient Ionization Mass Spectrometry[J].Analytical chemistry,201314(14):6545-6552. |
| [12] | Theodora Nah;ManNin Chan;Stephen R. Leone;Kevin R. Wilson.Real Time in Situ Chemical Characterization of Submicrometer Organic Particles Using Direct Analysis in Real Time-Mass Spectrometry[J].Analytical chemistry,20134(4):2087-2095. |
| [13] | 朱志强;闫建平;汪雨;陈瞬宗;陈焕文.表面解吸常压化学电离质谱快速鉴别樟木制品[J].分析化学,2013(6):905-910. |
| [14] | Bindesh Shrestha;Akos Vertes.High-Throughput Cell and Tissue Analysis with Enhanced Molecular Coverage by Laser Ablation Electrospray Ionization Mass Spectrometry Using Ion Mobility Separation[J].Analytical chemistry,20149(9):4308-4315. |
| [15] | David Calligaris;Diana Caragacianu;Xiaohui Liu;Isaiah Norton;Christopher J. Thompson;Andrea L. Richardson;Mehra Golshan;Michael L. Easterling;Sandro Santagata;Deborah A. Dillon;Ferenc A. Jolesz;Nathalie Y. R. Agar.Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis[J].Proceedings of the National Academy of Sciences of the United States of America,201442(42):15184-15189. |
| [16] | Zhigang Luo;Jiuming He;Yi Chen;Jingjing He;Tao Gong;Fei Tang;Xiaohao Wang;Ruiping Zhang;Lan Huang;Lianfeng Zhang;Haining Lv;Shuanggang Ma;Zhaodi Fu;Xiaoguang Chen;Shishan Yu;Zeper Abliz.Air Flow-Assisted Ionization Imaging Mass Spectrometry Method for Easy Whole-Body Molecular Imaging under Ambient Conditions[J].Analytical chemistry,20135(5):2977-2982. |
| [17] | Chen HW;Sun YP;Wortmann A;Gu HW;Zenobi R.Differentiation of maturity and quality of fruit using noninvasive extractive electrospray ionization quadrupole time-of-flight mass spectrometry[J].Analytical chemistry,20074(4):1447-1455. |
| [18] | Zhang, Hua;Chingin, Konstantin;Zhu, Liang;Chen, Huanwen.Molecular Characterization of Ongoing Enzymatic Reactions in Raw Garlic Cloves Using Extractive Electrospray Ionization Mass Spectrometry[J].Analytical chemistry,20155(5):2878-2883. |
| [19] | Jiangjiang Liu;He Wang;R. Graham Cooks;Zheng Ouyang.Leaf Spray: Direct Chemical Analysis of Plant Material and Living Plants by Mass Spectrometry[J].Analytical chemistry,201120(20):7608-7613. |
| [20] | 张华;朱亮;陈焕文.电喷雾内部萃取电离质谱直接分析蒜瓣组织的研究[J].分析化学,2014(11):1634-1639. |
| [21] | Hua Zhang;Liang Zhu;Liping Luo.Direct Assessment of Phytochemicals Inherent in Plant Tissues Using Extractive Electrospray Ionization Mass Spectrometry[J].Journal of Agricultural and Food Chemistry,201345(45):10691-10698. |
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