建立了液-液萃取后高效液相色谱法(HPLC)测定银杏叶提取物(EGb)中萜类内酯含量的快速方法.EGb样品溶于体积分数为30%的乙醇溶液后用乙醚萃取,有机相浓缩后的残留物以HPLC分析,组分的分离采用C18柱,以甲醇-水-磷酸(体积比为25:75:0.1)为流动相,示差检测器检测.结果表明,样品净化程序较已报道的方法更简便,选择性强、快速(少于20 min).该方法回收率大于99.0%、相对标准偏差小于2.0%、重现性好,可有效地用于银杏叶提取物的产品质量评价.
A new rapid analytical method was developed for the quantification of terpene lactones (bilobalide(BB) and ginkgolide A,B,C,J) in extract of Ginkgo biloba L. (EGb) using a liquid-liquid solvent extraction procedure followed by high performance liquid chromatography. EGb was dissolved in 30% ethanol and extracted with ether. After evaporation, the residue was then determinated by HPLC on a C18 column with methanol-water-orthophosphoric acid (25∶75∶0.1,V/V) as eluent and refractive index (RI) detection. Results showed that the excellent sample clean-up procedure is more simple and specific, and saves more time (less than 20 min) than any other methods that have been reported, and also leads to high recoveries (>99.0%) and low RSDs (<2.0%).The reproducible method is regarded to be very useful for evaluating the quality of extract of Ginkgo biloba L..
参考文献
| [1] | Van Beek T A, Lelyveld G P. Phytochem Anal,1993,4:109-114 |
| [2] | Lobstein-Guth A, Briancon-Scheid F, Anton R. J Chromatogr,1983,267:431-438 |
| [3] | Pietta P G, Mauri P L, Rava A. Chromatographia,1990,29:251-253 |
| [4] | Pietta P, Mauri P, Rava A. J Pharma & Biomed Anal,1992,10:1077-1079 |
| [5] | Van Beek T A, Scheeren H A, Rantio T, et al. J Chromatogr,1991,543:375-387 |
| [6] | Camponovo F F,Wolfender J L, Maillard M P, et al. Phytochem Anal,1995,6:141-148 |
| [7] | Hasler A, Meier B. Pharm Pharmacol Lett,1992,2:187-190 |
| [8] | Huh H, Staba E J. Planta Media,1993,59:232-239 |
| [9] | Chauret N, Carrier J, Mancini M, et al. J Chromatogr,1991,588:281-287 |
| [10] | Oehrle S A. J Liq Chromatogr,1995,18:2 855-2 859 |
| [11] | Van Beek T A,Van Veldhuizen A, Lelyveld G P, et al. Phytochem Anal,1993,4:261-268 |
| [12] | Steinke B, Muller B, Wanger H. Planta Media,1993,59:155-160 |
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