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利用反相高效液相色谱（RP-HPLC）和离子交换色谱（IEC）比较研究了固相化学合成的多肽类神经毒素虎纹捕鸟蛛毒素-Ⅰ（HWTX-Ⅰ）与其对应的天然产物的色谱行为差异及分离纯化方法。发现在完全相同的实验条件下，复性处理后的合成HWTX-Ⅰ的谱峰有别于还原变性后又复性的天然HWTX-Ⅰ的谱峰，主要表现为扩散度较大，对称性较差，说明合成产物存在更大分子构象的不均一性。推测除了在合成HWTX-Ⅰ复性过程中有少数分子发生二硫键错配外，还有部分分子在固相合成中发生了消旋作用，需要交替采取多种色谱法才能完全分离纯化复性后的合成多肽产物。

A comparative study was made to investigate the chromatographic behavior and purification of chemically synthesized and native polypeptide neurotoxin huwentoxin-Ⅰ(HWTX-Ⅰ) by means of reversed-phase HPLC and ion-exchange chromatography. The results showed that the synthetic HWTX-Ⅰ in crude product has a longer retention time than the native HWTX-Ⅰ,but has the same retention time as the denatured HWTX-Ⅰ, suggesting it being in a state of complete denaturation which was confirmed by the MALDI-TOF MS analysis. It has also been discovered that, before complete purification, the synthetic HWTX-Ⅰ, after oxidative treatment, showed wider and more asymmetric peaks than the native HWTX-Ⅰ that was denatured and then renatured under the same experimental conditions, which suggests that there is even greater conformational unhomogeneity in the synthetic HWTX-Ⅰ. It was inferred that, in addition to the wrong disulfide bonds formation during oxidative folding, the racemization during chemical synthesis resulted in more stereoisomers of synthetic HWTX-Ⅰ. It was needed for the renatured synthetic peptides to be purified using different methods alternatively.