以L-白氨酸为手性选择剂用毛细管电泳法拆分12种手性药物

色谱 , 2001, 19(2): 109-111. doi: 10.3321/j.issn:1000-8713.2001.02.004

以L-白氨酸为手性选择剂用毛细管电泳法拆分12种手性药物

林秀丽 ^1, , 李关宾 ^2, , 主沉浮 ^3, , 吴培 ^4, , 关亚风 ^5,

1.山东轻工业学院化工系;中国科学院大连化学物理研究所

2.山东轻工业学院化工系,

3.山东轻工业学院化工系,

4.山东轻工业学院化工系,

5.中国科学院大连化学物理研究所,

基金项目: 山东省自然科学基金(Y97B03026)

关键词：毛细管电泳法 , 手性分离 , L-白氨酸 , 手性药物

Enantioseparation of Twelve Pharmaceutical Racemates with High Performance Capillary Electrophoresis Using L-Leucine as Chiral Selector

建立了一种以L-白氨酸为手性选择剂用毛细管区带电泳法快速分离12种手性药物的方法。实验结果表明，手性对映体的分离度受L-白氨酸浓度和缓冲液pH的影响。在含有70 mmol/L L-白氨酸，50 mmol/L硼砂(pH 9．0)的溶液中，12种手性药物在11 min之内得到了基线分离。

A rapid enantiomeric separation method using L-leucine as chiral selector was established． Capillary zone electrophoresis (CZE) has been used for the enantiomeric separation of twelve pharmaceutical racemates with bare fused silica capillary and employing L-leucine as chiral selector． The enantiomeric resolution was influenced by L-leucine concentration and pH of background electrolyte (BGE)． The effects of the BGE types and concentrations on the enantiomeric separation were also investigated． The results showed that in the solution containing 50 mmol/L borax and 70 mmol/L L-leucine (pH 9.0), all the twelve drugs were on baseline separated in less than 11 minutes．

参考文献

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