采用柠檬酸-柠檬酸钠作为缓冲体系,使用负高压,对Cl-,NO3-,HCO3-和H2PO4-等4种常见阴离子进行了分离检测,研究了缓冲剂的种类、浓度、pH值及操作电压对分离的影响.在选定的条件下,4种离子的定量线性范围:Cl- 5.0×10-5 mol/L～2.5×10-3 mol/L,NO3- 6.0×10-5 mol/L～2.0×10-3 mol/L,HCO3- 5.0×10-6 mol/L～2.0×10-4 mol/L,H2PO4- 6.0×10-5 mol/L～1.0×10-3 mol/L;检出限:Cl- 1.5×10-5 mol/L,NO3- 3.0×10-5 mol/L,HCO3- 1.0×10-6 mol/L,H2PO4- 2.0×10-5 mol/L;峰面积的RSD(n=6):Cl- 3.1%,NO3- 3.3%,HCO3- 2.6%,H2PO4- 2.9%.并应用选定条件对自来水样品进行了分析.
Capillary electrophoresis with conductivity detection was used to separate and detect chloride (Cl-), nitrate (NO3-), bicarbonate (HCO3-) and dihydric phosphate (H2PO4-) ions. The proposed method was carried out by using citric acid and sodium citrate as buffer solution, under the condition of a -10 kV high voltage power supply. The effects of various buffers, concentrations, pH values and running voltages on separation were investigated. Under the chosen conditions, the linear ranges of Cl-, NO3-, HCO3-, and H2PO4- were 5.0×10-5 mol/L-2.5×10-3 mol/L, 6.0×10-5 mol/L-2.0×10-3 mol/L, 5.0×10-6 mol/L-2.0×10-4 mol/L and 6.0×10-5 mol/L-1.0×10-3 mol/L respectively. The detection limits were 1.5×10-5 mol/L, 3.0×10-5 mol/L, 1.0×10-6 mol/L, 2.0×10-5 mol/L and the relative standard deviations (RSD) of migration time were 3.1%, 3.3%, 2.6% and 2.9% respectively. Tap water was analyzed under the same conditions.
参考文献
| [1] | Jones W R, Jandik P. J Chromatogr, 1992, 608(1-2):395 |
| [2] | Jones W R, Jandik P. J Chromatogr, 1991, 546(1-2):445 |
| [3] | XIE Tian-yao, ZHENG Yi-ning, MO Jin-yuan, et al. Journal of Instrumental Analysis, 2000, 19(3):5谢天尧,郑一宁,莫金垣,等. 分析测试学报, 2000, 19(3):5 |
| [4] | Kuhn R, Stoecklin F, Erni F. Chromatographia, 1992, 33(1):32 |
| [5] | Issaq H J, Atamna I Z, Muschik G M. Chromatographia, 1991, 32(3-4):155 |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%