建立了快速分离和检测引起仔猪腹泻的肠毒性大肠杆菌K88、K99和987P细菌细胞的毛细管区带电泳方法,并进行了腹泻仔猪粪便中肠毒性大肠杆菌的应用检测分析.结果表明,在电泳缓冲液为0.05 mol/L Na2CO3-NaHCO3(pH 9.9)、分离电压为14.1 kV、检测波长为210 nm的电泳条件下,E.coli K88、K99和987P的细胞分别具有单一、稳定的特征谱峰,其保留时间的相对标准偏差RSD≤0.9%;在确定的实验条件下,实现了腹泻仔猪粪便中肠毒性大肠杆菌的快速检测分析,发现将出生5 d~6 d的腹泻仔猪粪便引入培养基中增殖后主要检出K88,将出生30 d~35 d的腹泻仔猪粪便引入培养基中增殖后主要检出K99,将出生60 d左右的腹泻仔猪粪便引入培养基中增殖后未发现肠毒性大肠杆菌.
参考文献
[1] | Schuett W, Thomaneck U, Knippel E, Rychly J, Klinkmann H. Electrophoresis, 1990, 11(11): 970 |
[2] | Hjerten S, Valtcheva L, Elenbring K, Eaker D. J Liq Chromatogr, 1989, 12: 2471 |
[3] | Hjerten S. Electrophoresis, 1990, 11: 665 |
[4] | Huang X, Horvath C. J Chromatogr A, 1997, 788: 155 |
[5] | Grossman P D, Soane D S. Anal Chem, 1990, 62: 1592 |
[6] | Armstrong D W, Schneiderheinze J M. Anal Chem, 2000, 72: 4474 |
[7] | Lund A, Hellemann A L, Vartdal F. J Clin Microbiol, 1988, 26(12): 2572 |
[8] | Duchet-Suchaux M, Bertin A, Dubray G. J Gen Microbiol, 1988, 134: 983 |
[9] | Ebersole R C, McCormick R M. Bio/Technology, 1993, 11: 1278 |
[10] | Armstrong D W, Schulte G, Schneiderheinze J M, Westenberg D J. Anal Chem, 1999, 71: 5465 |
[11] | Torimura M, Ito S, Kano K, Ikeda T, Esaka Y, Ueda T. J Chromatogr B, Biomed Sci Appl, 1999, 721(1): 31 |
[12] | Armstrong D W, Schneiderheinze J M, Kullman J P , He L F. FEMS Microbiol Lett, 2001, 194(1): 33 |
[13] | Stinson S C. Chem Eng News, 1999, 77: 36 |
[14] | Bundy J, Fensclau C. Anal Chem, 1999, 71: 1460 |
[15] | Longton R W, Cole Ⅲ J S, Quinn P F. Arch Oral Biol, 1975, 20: 103 |
[16] | Jaspers E, Overmann J. Appl Environ Microbiol, 1997, 63: 3176 |
[17] | Schneiderheinze J M, Armstrong D W, Schulte G, Westenberg D J. FEMS Microbiol Lett, 2000, 189(1): 39 |
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