采用分析型色谱饼对标准蛋白混合物进行了分离,结果表明装填有小颗粒填料的色谱饼在高流速条件下仍然具有良好的分离能力.在较大流速(5 mL/min)条件下,在10 min内对人血清白蛋白样品进行了快速纯化,其纯化后的人血清白蛋白的纯度大于85%,回收率为65%,说明分析型色谱饼可以用于快速分离纯化生物大分子.
参考文献
| [1] | Moore R M, Walters R R. J Chromatogr, 1984, 317: 119 |
| [2] | Liu Tong, Geng Xindu. Chinese Chemical Letters, 1999, 10(3): 219 |
| [3] | Geng Xindu, Regnier F E. J Chromatogr, 1984, 296:15 |
| [4] | Liu Tong, Geng Xindu. Journal of Northwest University(Natural Science Edition) (刘彤,耿信笃.西北大学学报(自然科学版)), 1999, 29(2): 123 |
| [5] | Zhang Yangjun, Li Xiang, Geng Xindu. Chinese Journal of Analytical Chemistry (张养军, 李翔, 耿信笃. 分析化学), 2002, 30 (5): 544 |
| [6] | Li Xiang, Zhang Yangjun, Geng Xindu. Chinese Journal of Chromatography (李翔, 张养军, 耿信笃. 色谱), 2002, 20(2): 133 |
| [7] | Geng Xindu. Guide to the Theory of Modern Separation Science. Beijing: Higher Education Press (耿信笃. 现代分离科学理论导引. 北京: 高等教育出版社), 2001. 382 |
| [8] | Cohn E J, Gurd F R N, Surgenor D M, Barnes B A, Brown R K, Derouaux G, Gillespie J M, Kahut F W, Lever W F, Liu C H, Mittean D, Mouton R F, Schmid K, Uroma E. J Am Chem Soc, 1950, 72 (1): 465 |
| [9] | Berglof J H, Eriksson S, Curling J M. J Appl Biochem, 1983, 5(4-5): 282 |
| [10] | Peters T, Taniuchi H, Anfinsen C B. J Biol Chem, 1973, 248: 2 447 |
| [11] | Wichman A, Andersson L O, Biochim Biophs Acta, 1974, 372: 218 |
| [12] | Shanbhag V P, Johansson G. Biochem J, 1976, 157: 301 |
| [13] | Saint Blacard J, Kurzin J M, Riberon P, Petit F, Fourcart J, Girot P N, Boschetti E. Anal Chem Sympser, 1982, 9: 305 |
| [14] | Su Zhiguo, Jiang Wei, Feng Pusun. Chinese Journal of Biotechnology (苏志国, 姜炜, 冯朴荪. 生物工程学报),1991, 7(2): 161 |
| [15] | Chang Jianhua, Geng Xindu,Yin Jianning. Chinese Journal of Chromatography(常建华, 耿信笃, 殷建宁. 色谱), 1991, 9(4): 263 |
| [16] | Ausubel F M, Brent R, Kingston R E, Moore D D, Seidman J G, Smith J A, Struhl K. Yan Ziying, Wang Hailin, Transl. Short Protocols in Molecular Biology. 3rd ed. Beijing: Science Press, 1998. 332 |
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