采用反相C18毛细管液相色谱柱,以乙腈(含0.1%(体积分数,下同)三氟乙酸)和水(含0.1%三氟乙酸)为流动相梯度洗脱,在26 min内分离了葛根异黄酮提取物以及愈风宁心片中的主要成分.采用毛细管液相色谱/四极杆飞行时间串联质谱仪对葛根提取物以及片剂中的几种主要异黄酮类化合物做了结构分析,发现葛根素是主成分(提取物中其平均质量分数是13.32% ;片剂中每片含量19.28～24.34 mg).对微量未知化合物,用它们的子离子谱图与已知化合物的谱图比较,推测其成分为3'-甲氧基葛根素和3'-甲氧基大豆苷.
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