采用高效液相色谱-二极管阵列检测/质谱(HPLC-DAD/MS)联用技术,以10 mmol/L醋酸铵和乙腈混合溶液梯度洗脱系统为流动相,应用C18色谱柱对生脉饮煎剂中人参皂甙类成分进行分离鉴定.分析结果表明:生脉饮煎剂中主要含有17个人参皂甙类成分,即20(R)-人参皂甙Rh1、Rh2、Rg3、Rg2,20(S)-人参皂甙Rh1、Rh2、Rg3、Rg2,人参皂甙Rf、Rg6、Rg5、F4、Rk1、Rk3、Rh4,20(S)-和20(R)-原人参三醇.人参皂甙成分在煎煮过程中发生了很大变化,主要变成了一些中低极性产物,这是因为煎煮过程中发生了水解、差向异构、脱水等反应.该方法简便、精确、灵敏度高,可以用来分析生脉饮煎剂中人参皂甙的变化.
参考文献
| [1] | Pharmacopoeia Commission of People's Republic of China.Pharmacopoeia of People's Republic of China:Part 1.Beijing:Chemical Industry Press (国家药典委员会.中华人民共和国药典:一部.北京:化学工业出版社),2005 |
| [2] | Tu Xiuhua,Tong Wenxin,Jiang Xifeng.Traditional Chinese Drug Research & Clinical Pharmacology (涂秀华,童文新,姜锡峰.中药新药与临床药理),2003,14(6):414 |
| [3] | Yan Yongqing.System Research of Sheng-Mai-San Oral Solution.Beijing:Chinese Medicine and Technology Publishing House (严永清.生脉散口服液的综合研究.北京:中国医药科技出版社),1990 |
| [4] | Tian Gengcun,Wang Aixiang.Chinese Traditional Patent Medicine (田更存,王爱香.中成药),2000,22(2):162 |
| [5] | Shen Liexing,Feng Xiao,Gao Xiuzhi.Herald of Medicine (沈烈行,冯晓,高秀芝.医药导报),2003,22(9):634 |
| [6] | Zhu Danni,Yan Yongqing,Li Zhiming.China Journal of Chinese Materia Medica (朱丹妮,严永清,李志明.中国中药杂志),1998,23(8):483 |
| [7] | Fuzzati N,Gabetta B,Jayakar K,Pace R,Peterlongo F.J Chromatogr A,1999,854:69 |
| [8] | Kite G C,Howes M J R,Leon C J,Simmonds M S J.Rapid Commun Mass Spectrom,2003,17:238 |
| [9] | Miao X S,Metcalfe C D,Hao C Y,March R E.J Mass Spectrom,2002,37:495 |
| [10] | Zhang H J,Wu Y J,Cheng Y Y.J Pharmacol Biomed Anal,2003,31:175 |
| [11] | Kwon S W,Han S B,Park I H,Kim J M,Park M K,Park J H.J Chromatogr A,2001,921:335 |
| [12] | Samukawa K,Yamashita H,Matsuda H,Kubo M.Chem Pharm Bull,1995,43:137 |
| [13] | Lau A J,Woo S O,Koh H L.J Chromatogr A,2003,1 011:77 |
| [14] | Lau A J,Seo B H,Woo S O,Koh H L.J Chromatogr A,2004,1 057:141 |
| [15] | Kim W K,Kim J M,Han S B,Lee S K,Kim N D,Park M K,Kim C K,Park J H.J Nat Prod,2000,63:1 702 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%