建立了采用超高效液相色谱同时测定化妆品中糖皮质激素、雌激素、雄激素、孕激素等共15种激素(曲安西龙、氢化可的松、泼尼松、可的松、甲基泼尼松龙、倍他米松、地塞米松、醋酸泼尼松龙、醋酸氢化可的松、雌三醇、雌二醇、雌酮、己烯雌酚、睾酮、孕酮)的分析方法.不同形态的化妆品样品均以甲醇为提取溶剂进行超声提取,经Oasis HLB固相萃取柱富集净化,以Waters ACQUITY UPLC BEH C18色谱柱(1.7 μm,2.1 mm×50 mm)分离,乙腈和水为流动相梯度洗脱,6 min内完成了15种激素的分离及检测.在1～25 ng进样范围内,15种激素的工作曲线的线性相关系数r均高于0.999 5.在低、中、高(2,10,20 mg/kg)3个添加水平下15种激素的平均回收率为88.2%～102.4%,相对标准偏差为1.6%～7.4%.
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