建立了液相色谱-质谱法测定织纹螺中河豚毒素的分析方法.匀质后的样品用0.03 mol/L乙酸溶液提取,水浴加热10 min,用Sep-Pak C18固相萃取柱净化,再用截留相对分子质量为3 000的超滤管过滤.采用Insertsil ODS-3色谱柱分离,以含有30 mmol/L七氟丁酸的甲酸铵溶液(1 mmol/L)-甲醇(体积比为99∶1)为流动相,采用电喷雾离子源,选择离子监测模式检测.以保留时间和河豚毒素的二级质谱特征碎片离子予以定性确证.结果表明,在此分析条件下,可将河豚毒素及其衍生物分离,在0.01~10.0 mg/L范围内线性关系良好(r2>0.995),检出限(以3倍信噪比为计)为2 μg/L,平均加标回收率为72.5%~80.4%,相对标准偏差为4.48%~8.87%.将该方法用于实际样品检测,在赤潮后所采集的织纹螺样品中检出了河豚毒素.
参考文献
[1] | Bulletin of Ministry of Health of P R China(中华人民共和国卫生部公告(2007年第10号)).[06-27-2007].http://www.moh.gov.cn/newshtml/18848.htm |
[2] | Jin L J,Hong R T,Ao Z X,Li J.Strail Journal of Preventive Medicine(金乐君,洪荣涛,敖志雄,李杰.海峡预防医学杂志),2002,8(6):64 |
[3] | Lin X T,Zhang M S,Zhang Y Y,Wang Z J,Sun X F,Xing J,Pang Z Q.Jiangsu Journal of Preventive Medicine(林祥田,张明生,张元瑛,王志坚,孙雪峰,邢军,庞中全.江苏预防医学),2005,16(2):5 |
[4] | Xu J Y,Xu G Z,Chen Y R,Qin P Z,Yu M,Ye L M,Yan P,Jin C G.Chinese Journal of Health Laboratory Technology (徐景野,许国章,陈耀荣,秦品章,于梅,叶鹿鸣,闫鹏,金春光.中国卫生检验杂志),2007,17(1):63 |
[5] | Jiang P Z.Foodborn Disease and Health.Beijing:Chemical Industry Press(姜培珍.食源性疾病与健康.北京:化学工业出版社),2006:144 |
[6] | Hwang D F,Chueh C H,Jeng S S.Toxicon,1990,28(1):21 |
[7] | Environmental Health Bureau,Ministry of Health and Welfare of Japan.Standard Methods of Analysis in Food Safety Regulation(Chemistry).Tokyo:Japan Food Hygiene Association,1991:296 |
[8] | Huang D F,Jeng S S.Journal of Chinese Biochemistry Society,1991,20:80 |
[9] | Narita H,Noguchi T,Maruyama J,Ueda Y,Hashimoto K,Watanabe Y,Hida K.Bulletin of the Japanese Society of Scientific Fisheries,1981,47:935 |
[10] | Wang J W,Luo X Y,Ji R,Zhang D,Zhang J.Journal of Hygiene Research(王健伟,罗雪云,计融,张镝,张靖.卫生研究),1997,26(2):106 |
[11] | Nagashima T,Maruyama J,Noguchi J,Hashimoto K.Nippon Suisan Gakkaishi,1987,53:819 |
[12] | Yasumoto T,Fudui M,Sasaki K,Sugiyama K.J AOAC Int,1995,78(2):574 |
[13] | Suenaga K,Kotoku S.Arch Toxicol,1980,44:291 |
[14] | Dell'Aversano C,Eaglesham G K,Quilliam M A.J Chromatogr A,2004,1 028:155 |
[15] | Shoji Y,Yotsu-Yamashita M,Miyazawa T,Yasumoto T.Anal Biochem,2001,290:10 |
[16] | Pharmaceutical Society of Japan.Standard Methods of Analysis for Hygienic Chemists-With Commentary.Zhang H X,transl.Beijing:Huawen Press(日本药学会编著.卫生试验法·注解.张洪祥,译.北京:华文出版社),1995:336 |
[17] | Yang C,Su X R,Li T W,Jiang S.Natural Product Research and Development(杨春,苏秀榕,李太武,蒋双.天然产物研究与开发),2003,15(5):398 |
[18] | Liu H X,Zhang N,Dong L M,Li L F.Journal of Fisheries of China(刘海新,张农,董黎明,李庐峰.水产学报),2006,30(6):812 |
[19] | Huang P C.Modern Preventive Medicine(黄浦昌.现代预防医学),1999,26(4):441 |
[20] | Lin X J,Xu W P,Jiang X G.Chinese Journal of Food Hygiene(林香娟,徐卫平,蒋贤根.中国食品卫生杂志),2000,12(1):53 |
[21] | Huang S G.Practical Preventive Medicine(黄曙光.实用预防医学),2003,10(1):85 |
[22] | Hwang D F,Cheng C A,Tsai H T,Shih D Y C,Ko H C,Yang R Z,Jeng S S.Fish Sci,1995,61(4):675 |
[23] | Hwang P A,Tsai Y H,Deng J F,Cheng C A,Ho P H,Hwang D F.J Food Prot,2005,65(1):1 696 |
[24] | Noguchi T J,Maruyama J,Narita H,Hashimoto K.Toxicon,1984,22:219 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分:
-
10%
-
20%
-
30%
-
40%
-
50%