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  4. 以亲水作用色谱为核心的液相色谱联用技术及其应用研究

色谱 , 2008, 26(6): 649-657. doi: 10.3321/j.issn:1000-8713.2008.06.001

以亲水作用色谱为核心的液相色谱联用技术及其应用研究

王媛 1, , 顾惠新 2, , 路鑫 3, , 许国旺 4,

1.中国科学院大连化学物理研究所分离分析化学开放实验室,辽宁,大连,116023;Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathtology,Faculty of Medicine and Dentistry,University of Alberta,Edmonton,AB,T6G 2G3,Canada

2.中国石油兰州石油化工公司兰港石化有限公司,甘肃,兰州,730060

3.中国科学院大连化学物理研究所分离分析化学开放实验室,辽宁,大连,116023

4.中国科学院大连化学物理研究所分离分析化学开放实验室,辽宁,大连,116023

基金项目:   科技部国家科技支撑计划(2006BAK02A12)   国家高技术研究发展计划(863计划)(2006AA02Z342)   中国科学院知识创新基金(2006A13)  

关键词： 亲水作用色谱 , 联用技术 , 柱切换 , 多维分离

Development of hydrophilic interaction chromatographic hyphenated techniques and their applications

亲水作用色谱(HILIC)是近年来色谱领域研究的热点之一.本文围绕复杂体系样品中亲水性组分的分离分析,综述了国际上近年来发展的以HILIC为核心的多种液相色谱联用技术及其应用.简要介绍了HILIC的起源、定义、分离特点及其常用固定相;比较了HILIC和反相色谱(RPLC)的选择特性;针对不同层次的分离对象和分离要求,讨论了多种基于HILIC的液相色谱以及液相色谱-质谱联用技术的分离特点和适用范围.

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