应用超高效液相色谱-串联质谱(UPLC-MS/MS)联用技术,建立了高灵敏、快速地同时检测农产品中8种种衣剂农药残留量的方法.样品经甲醇-水(体积比为1:1)提取后,无需经过任何净化过程;以梯度流动相洗脱、经Acquity UPLC C18超高效液相色谱柱分离;电喷雾正离子(ESI+)采集模式、多反应监测模式(MRM)对定量离子和定性离子进行MS/MS测定.8种种衣剂农药在0.001～0.20 mg/L范围内线性关系良好(r≥0.997).添加浓度为0.006～1.2 mg/kg时,回收率为60%～110%,相对标准偏差小于10%;方法的检出限为0.000 5～0.002 mg/kg.该方法仅需约2 min的检测时间,而且灵敏、准确,适合于水果、蔬菜、粮谷等农产品中种衣剂农药残留量的快速、高灵敏地检测分析.
参考文献
| [1] | Wu X H,Zhang W H,Liu P F.Plant Protection Technology and Extension (吴学宏,张文华,刘鹏飞.植保技术与推广),2003,23(10):36 |
| [2] | Zhang H H,Shi W Y.Seed (张红辉,石伟勇.种子),2002(2):39 |
| [3] | Du G L,Chi G T.Chinese Agricultural Science Bulletin (杜光玲,赤国彤.中国农学通报),2002,18(1):52 |
| [4] | Lin W X.The compilation of residue limits standards for pesticides and veterinary drugs in foodstuffs in the world.Dalian:Dalian Maritime University Press (林维宣.各国食品中农药兽药残留限量规定.大连:大连海事大学出版社),2002 |
| [5] | Ge Z R.Maximum residue limits of agricultural chemicals in foods (Japanese positive list system).Beijing:Standards Press of China (葛志荣.食品中农业化学品残留限量(药品卷日本肯定列表制度).北京:中国标准出版社),2006 |
| [6] | Zhang Z G,Zheng X H,Shi B,et al.Inspection and Quarantine Science (张志刚,郑向华,施冰,等.检验检疫科学),2004,14(B12):6 |
| [7] | Stajnbaher D,Zupancic-Kralj L.J Chromatogr A,2003,1 015:185 |
| [8] | Li Y,Zhou Y M,Niu S.Modern Scientific Instruments (李英,周艳明,牛森.现代科学仪器),2005(6):68 |
| [9] | Liao G H,Duan T T,Qin L X,et al.Guizhou Agricultural Sciences (廖国会,段婷婷,秦立新,等.贵州农业科学),2005,33(4):75 |
| [10] | Chen X M,Hu B Z,Liu H S,et al.Chinese Journal of Analytical Chemistry (陈笑梅,胡贝贞,刘海山,等.分析化学),2007,35(1):106 |
| [11] | Xie W,Ding H Y,Jiang X Y,et al.Chinese Journal of Chromatography (谢文,丁慧瑛,蒋晓英,等.色谱),2006,24(6):633 |
| [12] | Jia Y,Zhao P L.Physical Testing and Chemical Analysis Part B:Chemical Analysis (贾云,赵鹏林.理化检验:化学分册),2005,41(11):858 |
| [13] | Liu X S,Tong Z F,Zheng L,et al.Journal of Analytical Science (刘晓松,童张法,郑玲,等.分析科学学报),2007,23(3):311 |
| [14] | Melo L F,Collins C H,Jardim I C.J Chromatogr A,2005,1 073:75 |
| [15] | Liu C L,Liu F M,Li L,et al.Chinese Journal of Pesticide Science (刘承兰,刘丰茂,李莉,等.农药学学报),2004,6(4):93 |
| [16] | Lin J L,Zhang L J,Zhang X L,et al.Chinese Journal of Analytical Chemistry (林江丽,张丽静,张旭龙,等.分析化学),2001,29(8):933 |
| [17] | Fernandez M,Pico Y,Manes J.Chromatographia,2003,58:151 |
| [18] | Liu C W,Liu X W,Zhai G S.Chinese Journal of Chromatography (刘长武,刘潇威,翟广书.色谱),2003,21(3):255 |
| [19] | Jansson C,Pihlstrom T,Osterdahl B G,et al.J Chromatogr A,2004,1 023:93 |
| [20] | Hennion M C,Cau-Dit-Coumes C,Pichon V.J Chromatogr A,1998,823:147 |
| [21] | Bernal J L,Del Nozal M J,Toribio L,et al.J Chromatogr A,1997,787:129 |
| [22] | Pan J,Xia X X,Liang J.Ultrason Sonochem,2008,15(1):25 |
| [23] | GB/T 20755-2006 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%