建立了白首乌中4种苯乙酮类化合物(Ⅰ:4-羟基苯乙酮,Ⅱ:2,5-二羟基苯乙酮,Ⅲ:白首乌二苯酮,Ⅳ:2,4-二羟基苯乙酮)的高效液相色谱检测方法.采用Symmetry-C18色谱柱(4.6 mm × 250 mm,5μm)分离,流动相为甲醇-水(体积比为26:74),流速1.0 mL/min;采用二极管阵列检测器检测,化合物Ⅰ、Ⅲ、Ⅳ的检测波长为280nm,化合物Ⅱ的检测波长为224 nm;测定温度为30℃.4种苯乙酮类化合物Ⅰ～Ⅳ的线性范围分别为0.080～0.560 μg,0.080～0.560 μg,0.100～0.700μg和0.092～0.644μg(r=0.999 6～0.999 9);平均加样回收率(n=3)为98.0%～104.0%,相对标准偏差为0.8%～2.6%.该方法简便快速、结果准确、重现性好,可作为白首乌药材质量控制的一个有效方法.
参考文献
| [1] | Xiao P G.Records of Chinese traditional medicine (New Edition).Beijing:Chemical Industry Press (肖培根.新编中药志.北京:化学工业出版社),2002:525 |
| [2] | Xu L C,Zhang H,Xu C S.Study Journal of Traditional Chinese Medicine (徐凌川,张华,许昌盛.中医药学刊),2003,21(11):1893 |
| [3] | Zhang R S,Ye Y P,Liu X L.Chinese Traditional and Herbal Drugs (张如松,叶益萍,刘雪莉.中草药),2000,31(8):599 |
| [4] | Song J M,Ding X L.Food Science (宋俊梅,丁霄霖.食品科学),1997,18(9):61 |
| [5] | Gao L J,Wang J H,Cui J H,et al.China Journal of Chinese Materia Medica (高丽君,王建华,崔建华,等.中国中药杂志),2005,30(17):352 |
| [6] | Li J,Kadota S,Kawata Y,et al.Chemical Pharmaceutical Bulletin,1992,40(12):3 133 |
| [7] | Lin A Q.[Master dissertation],Jinan:Shandong University of Traditional Chinese Medicine (林爱群.[硕士学位论文].济南:山东中医药大学),2005 |
| [8] | ShengL S,Wang Y,Ma R L,et al.Chinese Journal of Chromatography (盛龙生,王颖,马仁玲,等.色谱),2003,21 (6):549 |
| [9] | Wang Z L,Wang H,Chen S Z.Chinese Journal of Chromatography (王占良,王弘,陈世忠.色谱),2003,24(4):325 |
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