建立了用荧光检测器同时测定11种喹诺酮类药物(包括诺氟沙星、培氟沙星、环丙沙星、恩诺沙星、氧氟沙星、达氟沙星、洛美沙星、二氟沙星、沙拉沙星、恶喹酸和氟甲喹)在鸡肉中的多残留的高效液相色谱检测方法.鸡肉样品用10%三氯乙酸-乙腈(体积比为7∶3)提取两次并稀释,随后用反相固相萃取柱净化.采用Hypersil BDS-C18色谱柱分离,以乙腈和水为流动相梯度洗脱,荧光检测器用程序编程检测波长检测.11种喹诺酮类药物标准曲线的线性范围为5～1200 μg/L,相关系数大于0.998.在高、中、低三个添加水平下的回收率为56% ～119% ,批内相对标准偏差为0.4% ～16.1% ,批间相对标准偏差为1.4% ～23.0% .检出限和定量限分别为1～23 μg/kg和4～40 μg/kg.该方法快速、灵敏,达到了兽药残留检测的要求.
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