采用水包油型微乳液相色谱(MELC)分离了6种激素类药物(醋酸可的松、泼尼松龙、己烯雌酚、炔雌醇、醋酸氟轻松及黄体酮).考察了微乳流动相的组成成分(包括表面活性剂的浓度、油相种类、有机添加剂种类)及固定相孔径等对分离的影响.实验得到的最佳分离条件:色谱柱为Venusil ASB C18 (T)(粒径5 μm,孔径30 nm,250 mm×4.6 mm),微乳流动相为30 g/L 十二烷基硫酸钠(SDS)-0.8%正辛烷-6.6%正丁醇,流速为0.8 mL/min,检测波长为254 nm,柱温为35 ℃.该方法可用于甾体药物及其制剂的分离鉴别以及快速测定.
参考文献
| [1] | Berthod A,Garcia-Alvarez-Coque C.Micellar Liquid Chromatography.[S.l.]:CRC Press,2000 |
| [2] | Berthod A,De Carvalho M.Anal Chem,1992,64:2267 |
| [3] | Berthod A,Lasema J,Carretero L.J Liq Chromatogr,1992,15:3115 |
| [4] | Marsh A,Clark B,Altria K.Chromatographia,2004,59:531 |
| [5] | El-Sherbiny D T M,El-Ashry S M,Mustafa M A,et al.J Sep Sci,2003,26:503 |
| [6] | Marsh A,Clark B J,Altria K D.Chromatographia,2005,61(11/12):539 |
| [7] | El-Sherbiny D T,El-Enany Nahed,Belal F F,et al.J Pharmaceut Biomed Anal,2007,43(4):1236 |
| [8] | Jancic B,Medenica M,Ivanovic D,et al.Anal Bioanal Chem,2005,383:687 |
| [9] | Malenovic A,Ivanovic D,Medenica M,et al.J Chromatogr A,2006,1131(1/2):67 |
| [10] | Malenovic A,Ivanovic D,Medenica M,et al.J Sep Sci,2004,27:1087 |
| [11] | He S,Zhang Z Y,Zhang S Y.Journal of Instrumental Analysis (贺帅,张忠义,张守尧.分析测试学报),2008,27(6):654 |
| [12] | Jancic B,Ivanovic D,Medenica M,et al.J Chromatogr A,2005,1088:187 |
| [13] | Torres Cartas S,Garcia Alvarez-Coque M C,Villanueva Camanas R M.Anal Chim Acta,1995,302:163 |
| [14] | The Pharmacopoeia Committee of People's Republic of China.Pharmacopoeia of People's Republic of China,Part Ⅱ.Beijing:Chemical Industry Press (国家药典委员会.中华人民共和国药典,二部.北京:化学工业出版社),2000:369,643 |
| [15] | David P T,Joe P F.J Chromatogr A,2004,1060:195 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%