研究了从白花蛇舌草水提取物中选择性富集环烯醚萜苷类成分的方法.该方法采用硅胶基质的寡聚乙二醇(OEG,实验室自合成)和ODS两种填料依次作为固定相,对白花蛇舌草水提醇沉样品进行固相萃取,并以超高效液相色谱(UPLC)系统对在富集的各个阶段得到的产物进行了色谱表征.实验结果表明,采用该方法得到的终产物的产率为8.21% .从UPLC谱图中可以看出固相萃取环烯醚萜苷类成分选择性富集的过程.终产物中14种典型的环烯醚萜苷类化合物含量明显升高,可达白花蛇舌草水提物的6.1倍,回收率为50.1% ,富集效果明显.因此,将白花蛇舌草水提物醇沉后依次经过OEG柱与ODS柱的串联固相萃取可选择性地富集环烯醚萜苷类成分.该方法操作步骤较少,操作简便,选择性好,提取效率较高,富集效果明显.
参考文献
| [1] | The Editorial Committee on the Chinese Materia Medica of the State Administration of Traditional Chinese Medicine.Chinese materia medica:Vol 6.Shanghai:Shanghai Science and Technology Publishers (国家中医药管理局中华本草编委会.中华本草:第6册.上海:上海科学技术出版社),1998:433 |
| [2] | Huang J R,Liu Y H,Yu Z B,et al.Chinese Traditional Patent Medicine (黄建荣,刘咏海,喻志标,等.中成药),2005,27(11):1 329 |
| [3] | Wu K S,Zhang K,Tan G S,et al.Chinese Pharmaceutical Journal (吴孔松,张坤,谭桂山,等.中国药学杂志),2005,40(11):817 |
| [4] | Yang Y B,Yang X Q,Ding Z T.Journal of Yunnan University:Natural Sciences Edition (杨亚滨,杨雪琼,丁中涛.云南大学学报:自然科学版),2007,29(2):187 |
| [5] | Zhang H J,Chen Y G.Journal of Yunnan Normal University (张海娟,陈业高.云南师范大学学报),2006,26(3):65 |
| [6] | Si J Y,Chen D H,Pan R L,et al.Chinese Traditional and Herbal Drugs (斯建勇,陈迪华,潘瑞乐,等.中草药),2008,39(4):507 |
| [7] | Liang Z T,Jiang Z H,Leung K S Y,et al.Chem Pharm Bull,2006,54(8):1 131 |
| [8] | Kim D H,Lee H J,Oh Y J,et al.Arch Pharm Res,2005,28(10):1 156 |
| [9] | Kim Y,Park E J,Kim J,et al.J Nat Prod,2001,64(1):75 |
| [10] | Li C M,Zhang X L,Xue X Y,et al.Rapid Comm Mass Spectrom,2008,22:1 941 |
| [11] | Liu K,Yan L Q,Yao G C,et al.J Chromatogr B,2006,831:303 |
| [12] | Yao G,He Z Y,Fang J N.Chinese Traditional and Herbal Drugs (姚干,何宗玉,方积年.中草药),2006,37(1):57 |
| [13] | Wang B,Zheng F,Guo L,et al.Natural Product Research and Development (王冰,郑凤,郭雷,等.天然产物研究与开发),2006,18:117 |
| [14] | Li M X,Jia Z P,Hu Z D,et al.China Journal of Chinese Materia Medica (李茂星,贾正平,胡之德,等.中国中药杂志),2007,32(17):1 743 |
| [15] | Ding X,Yu Z L,Du W F,et al.Chinese Traditional Patent Medicine (丁霞,余宗亮,杜伟峰,等.中成药),2008,30(1):53 |
| [16] | Cai Q,Liu T,Fu Y Q,et al.Chinese Traditional Patent Medicine (才谦,刘涛,付玉芹,等.中成药),2003,25(4):271 |
| [17] | Li C M,Xu Q,Xue X Y,et al.World Science and Technology:Modernization of Traditional Chinese Medicine and Materia Medica (李存满,徐青,薛兴亚,等.世界科学技术:中药现代化),2007,9(4):51 |
| [18] | Fu R N.Chinese Journal of Analysis Laboratory (傅若农.分析试验室),2007,26(2):100 |
| [19] | Mroczek T,Glowniak K,Kowalska J.J Chromatogr A,2006,1 107:9 |
| [20] | Batt A L,Kostich M S,Lazorchak J M.Anal Chem,2008,80:5 021 |
| [21] | Guo Z M,Liu Y F,Xu J Y,et al.J Chromatogr A,2008,1 191(1/2):78 |
| [22] | Liu Y M,Guo Z M,Jin Y,et al.J Chromatogr A,2008,1 206(2):153 |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%