色谱-质谱联用技术在代谢组学中的应用

色谱 , 2009, 27(5): 566-572. doi: 10.3321/j.issn:1000-8713.2009.05.007

色谱-质谱联用技术在代谢组学中的应用

黄强 ^1, , 尹沛源 ^2, , 路鑫 ^3, , 孔宏伟 ^4, , 许国旺 ^5,

1.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

2.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

3.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

4.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

5.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

基金项目: 国家自然科学重点基金面上基金(20675082) 国家自然科学基金重点项目(20835006)

关键词：色谱 , 质谱 , 代谢组学 , 代谢谱

Applications of chromatography-mass spectrometry in metabonomics

代谢组学是对生物体受外部刺激所产生的小分子代谢产物的变化或其随时间的变化进行研究的一门学科,以实现对体液、细胞以及组织提取物等复杂的生物样本中所有代谢产物的定性和定量分析为研究目标.色谱-质谱联用技术在代谢组学的研究中已显示出极大的发展潜力.本文主要综述近年来代谢组学研究中涉及的色谱-质谱联用技术及其数据处理方法,重点介绍各种分离技术的特点及其在应用中的关键问题,并对其在代谢组学应用中的未来发展给予展望.

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