毛细管电色谱和加压毛细管电色谱的进展与应用

色谱 , 2009, 27(5): 609-620. doi: 10.3321/j.issn:1000-8713.2009.05.012

毛细管电色谱和加压毛细管电色谱的进展与应用

吴漪 ^1, , 张晓晖 ^2, , 魏娟 ^3, , 薛云云 ^4, , 玛尔江·巴哈提别克 ^5, , 王彦 ^6, , 阎超 ^7,

1.上海交通大学药学院,上海,200240

2.上海交通大学药学院,上海,200240

3.上海交通大学药学院,上海,200240

4.上海交通大学药学院,上海,200240

5.上海交通大学药学院,上海,200240

6.上海交通大学药学院,上海,200240

7.上海交通大学药学院,上海,200240

基金项目: 上海市科委科技创新行动计划(08142201600) 科技部农业技术成果转化资金项目(083219N8300)

关键词：毛细管电色谱 , 加压毛细管电色谱 , 固定相 , 生物分析 , 药物分析 , 食品分析 , 环境分析

Recent advances and applications of capillary electrochro-matography and pressurized capillary electrochromatography

毛细管电色谱(CEC)以内含色谱固定相的毛细管为分离柱,以电渗流为驱动力,既可以分离带电物质也可以分离中性物质.它结合了毛细管电泳和高效液相色谱两者的优点,兼具高柱效、高分辨率、高选择性和高峰容量的特点,同时具有色谱和电泳的双重分离机理.然而,"纯粹"的电色谱在实际应用中有着天然的弱点,即:在电流通过毛细管柱中的流动相时容易产生气泡(焦耳热作用),从而使电流中断和电渗流停止,毛细管柱必须被重新用流动相润湿后方能再次使用.加压毛细管电色谱(pCEC)将液相色谱中的压力流引入CEC系统中,不仅解决了气泡、干柱等问题,而且实现了定量阀进样和二元梯度洗脱.CEC和pCEC作为微分离领域的两种前沿技术,满足了当前复杂样品分析和分析仪器微型化的需求,近年来获得了广泛的关注.本文综述了这两种技术近来的发展,包括仪器、色谱固定相的发展,总结了其在生命科学、药物分析、食品安全以及环保样品分析等方面的应用进展,评述了各方法的特点,并展望了CEC和pCEC今后的发展和应用前景.

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