生物玻璃矿化性能及其离子溶出对成骨细胞功能的影响

无机材料学报, 2007, 22(1): 123-127. doi: 10.3724/SP.J.1077.2007.00123

生物玻璃矿化性能及其离子溶出对成骨细胞功能的影响

华南理工大学1. 生物材料研究所

2. 2. 特种功能材料及其制备新技术教育部重点实验室, 广州 510640

3. 3. 南方医科大学解剖学教研室, 广州 510515

4. 4. 广东省组织构建与检测重点实验室, 广州 510515

South China University of Technology 1. Institute of Biomedical Material

2. 2. Key Laboratory of Specially Functional Materials and Advanced Manufacturing Technology of Ministry of Education, Guangzhou 510640, China

3. 3. Department of Anatomy, Southern Medical University, Guangzhou 510515, China

4. 4. Key Laboratory of Tissue Construction and Detection of Guangdong, Guangzhou 510515, China>

关键词：生物玻璃 , bio-mineralization , cellular viability , alkaline phosphatase

Bio-mineralization Properties of Bioglass and Effect of Ionic Products from Bioglass Dissolution on Osteoblast Functions

TU Jie , WANG Ying-Jun , CHEN Xiao-Feng , OU Yang-Jun , QIU Xiao-Zhong

Keywords： bioglass , 生物矿化性能 , 细胞增殖 , 碱性磷酸酶

通过体外矿化实验, 对比研究了熔融法生物玻璃45S5和溶胶－凝胶法生物玻璃58S、77S的生物矿化性能的差异;并运用了这三种生物玻璃的DMEM(Dulbecco’s Modified Eagle Medium)浸提液对MC-3T3细胞进行培养, 以考察不同生物玻璃在培养液中的离子溶出对成骨细胞增殖及ALP(碱性磷酸酶)活性的影响. 通过体外矿化实验可以发现, 77S的生物矿化性能相
对45S5和58S较差, 在SBF溶液(模拟体液)中浸泡96h只能形成HA(羟基磷灰石)晶体, 45S5和58S则能生成类骨HCA(碳酸羟基磷灰石)晶体; 但细胞培养实验发现, 77S浸提液中培养的细胞, 其细胞增殖和ALP活性都明显高于45S、58S和对照组. 由此认为, 一定的离子浓度范围内, Si离子的存在有利于成骨细胞的增殖与分裂.

The bio-mineralization properties of the melt-derived bioglass 45S5 and the sol-gel derived bioglass 58S and 77S in vitro were investigated. The effect of ionic products from bioactive glass dissolution on osteoblast proliferation and ALP activity was also discussed. The results show that osteoblasts cultured with 77S conditioned DMEM show better cellular viability and ALP activity when compared to 45S5 and 58S, though the bio-mineralization of 77S shows a little worse---only HA can form from 77S when HCA can form from 45S5 and 58S. By comparing the Si concentrations in bioglass conditioned DMEM and control DMEM, it can be concluded that solutions containing high Si concentrations in certain range are mitogenic for bone cells.

参考文献

[1]

Hench L L. Science, 2002, 295: 1014--1017.
[2] Hench L L. Bioceramics, 1998, 1: 1705--1728.
[3] Rámila A, Regí M V. Biomaterials, 2001, 22: 2301--2306. [4] Burg K J, Porter S, Kellam J F. Biomaterials, 2000, 21: 2347--2359.
[5] Hench L L, Xynos I, Edgar A, et al. 无机材料学报(Journal of Inorganic Materials), 2002, 17 (5): 897--909.
[6] Xynos I, Edgar A J, Buttery L, et al. Biochem. Biophys. Res. Commun., 2000, 276 (2): 461--465.
[7] Hattar S, Asselin A, Greenspan D, et al. Biomaterials, 2005, 26: 839--848.
[8] 张梅梅, 陈晓峰, 王迎军, 等. 玻璃与搪瓷, 2003, 31 (3): 15--20.
[9] 陈晓峰, 张梅梅, 赵靖, 等. 生物医学工程学杂志, 1999, 16 (?
增刊): 25--28.
[10] Liu Xuanyong, Ding Chuanxian. Materials Letter, 2002, 57 (3): 652--655.
[11] 肖斌, 周大利, 杨为中(XIAO Bin, et al).
无机材料学报(Journal of Inorganic Materials), 2006, 21 (2): 427--432.
[12] 王传辉, 李贺军, 黄凤萍(WANG Chuan-Hui, et al). 无机材料学报(Journal of Inorganic Materials), 2005, 20 (4): 837--841.
[13] 钟吉品(ZHONG Ji-Pin). 无机材料学报(Journal of Inorganic Materials), 1995, 10 (2): 129--137.
[14] 张克从, 张乐. 晶体生长科学与技术, 第二版. 北京: 科学出版社, 1997. 221--228.

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