为了评价不同形貌的纳米羟基磷灰石粒子(nano-HA)对脑胶质母瘤细胞系U87细胞的作用,合成了三种不同形貌的nano-HA,与U87细胞共培养,培养基中同时还添加了聚乙二醇(PEG),以保持nano-HA的稳定性.透射电镜(TEM)及动态光学散射(DLS)测定高度分散在培养基中nano-HA的稳定情况和粒子大小,MTT法检测了nano-HA对U87细胞的作用.实验结果表明,nano-HA可显著抑制U87细胞增殖;并且,针状纳米粒子的抑制能力要强于另外两种粒子.此外,通过RT-PCR检测发现不同形貌的nano-HA可不同程度的下调细胞的Toll样受体4(TLR4)的表达水平,这可能是其具有不同生物学活性的原因之一
To evaluate the effects of Hydroxyapatite nano-particles(nano-HA)with different morphologies on human U87 glio blastoma cell line(U87)，three kinds of nano-HA were designed and synthesized，and a co-culture system containing nano-HA and U87 cells was established．Polyethylene glycol(PEG)was also added to the HA medium system for the samples tabiliza tion,After determining the stabilization and high dispersed nanometer size of nano-HA in the medium system using Transmission electron microscope(TEM)and dynamic light scattering(DLS),the effects of narlo-HA on GBM celts were assessed with an MTr method.Results showed that nano-HA could effectively inhibit U87 cells roliferation.Compared ith other nano-HA,the needle-like nano-HA were more powerful in inhibiting U87 cells proliferation.Toll-like receptor 4(TLR4)induced by different nano-HA may be one reason for their different bioactivity.
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