支架孔隙贯通性的研究一直是多孔生物陶瓷的研究重点之一.采用石蜡球作为造孔剂, 在常规的颗粒造孔法制备多孔陶瓷支架的基础上,通过二甲苯处理以便在石蜡球间形成桥联结构, 以扩大颗粒间的接触面积,从而提高多孔陶瓷支架的孔隙贯通性. 借助扫描电镜(SEM)观察陶瓷支架的多孔结构,评价二甲苯处理石蜡球对陶瓷支架孔隙贯通性的改善效果; 采用密度法测定了陶瓷支架的孔隙率并计算其收缩率,并用成骨细胞评价陶瓷支架的细胞相容性. 结果表明,通过二甲苯的处理, 不仅改善了陶瓷支架孔隙的贯通性,而且提高了其孔隙率, 但孔隙率对陶瓷支架的收缩率无明显影响.细胞培养实验显示成骨细胞可进入多孔陶瓷支架内部, 并在材料表面正常生长,贯通性好的多孔陶瓷支架可为成骨细胞生长提供更充分的空间.
The pore interconnectivity isa key factor for the application of porous bioceramics as bone tissue engineeringscaffolds. Packed wax spheres were treated with dimethyl benzene and acted as theporogen for the preparation of porous ceramic scaffolds, in order to increasethe inter-sphere connectivity. The morphology, porosity, shrinkage, and thecompressive strength of the obtained ceramic scaffolds were analyzed. The cellcompatibility of the scaffolds was evaluated with Sprague-Dawley (SD) osteoblasts.The results showed that the pore interconnectivity and volumetric porosity weresignificantly increased by the dimethyl benzene treatment. Scaffolds with differentporosities had similar shrinkage. Osteoblasts migrated deeply into the centerof the porous ceramic scaffold through the interconnected pores and got normalactivity. Porous scaffolds with better interpore connectivity could provid morespace for the proliferation of osteoblasts.
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