研制具有药物缓释功能的骨组织工程支架, 对载药微球包埋于羟基磷灰石/聚氨酯(HA/PU)支架中的药物缓释体系进行了可行性研究. 首先将盐酸环丙沙星作为模型药物, 包裹于乙基纤维素(EC)微球中, 然后将EC微球与HA/PU材料进行复合, 制备了抗生素药物缓释支架. 结果显示EC微球均匀地分布在HA/PU支架基质中, 未对支架的开孔结构和孔隙形貌构成影响. 与单纯将药物载入HA/PU支架中相比, 复合载药EC微球的HA/PU支架的初期药物暴释明显降低, 药物缓释时间延长. 体外药物释放实验和抑菌实验结果表明, 该载药微球支架具有良好的药物缓释功能和抑菌性能, 是一种集骨修复和治疗于一体的新型组织工程支架材料.
To explore and develop scaffold for bone regeneration or tissue engineering with the capacity of controlled drug delivery, the feasibility of hydroxyapatite/polyurethane (HA/PU) scaffold containing drug-loaded microspheres for controlled drug delivery system was demonstrated. Ciprofloxacin hydrochlorid as a model drug was encapsulated in ethyl cellulose (EC) microspheres, which were subsequently incorporated into HA/PU composite scaffold to generate an antibiotic drug delivery system. The results show that EC microspheres are uniformly distributed in the HA/PU scaffold matrix and display no significant effect on the pore structure of the scaffold. Compared with incorporating ciprofloxacin hydrochlorid into scaffolds directly, embedding microspheres into scaffolds significantly reduces the initial burst drug release and extends the release time of drug delivery. In vitro drug delivery tests and antibacterial activity tests prove that drug-loaded microsphere/scaffold system has good drug delivery properties and effective antibacterial properties. These results suggest that the novel drug-loaded microsphere/ scaffold composites developed in this study is a good candidate scaffold with the function of bone repair and infection treatment for bone tissue engineering.
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