通过阳极氧化技术,在钛表面制备一层管径为100nm左右的氧化钛纳米管.选取小牛血清白蛋白(BSA)和纤维连接蛋白(FN)两种蛋白质进行蛋白吸附实验,并在仿生条件下进行蛋白质的体外释放.对吸附了蛋白的纳米管试样表面进行红外和荧光定性分析,同时采用考马斯亮蓝法对纳米管表面的蛋白吸附进行定量检测,实验发现纳米管试样更有利于蛋白质的吸附,且FN在试样表面吸附时的吸附率大于BSA.氧化钛纳米管的蛋白释放分为突释和缓释两个阶段,其释放机制符合Fickian扩散.
参考文献
| [1] | 刘达理,冯波,鲁雄,等(LIU Da-Li,et al).两段式阳极氧化法制备大管径TiO2纳米管.稀有金属材料与工程(Rare Metal Mat.Eng.),2010,39(2):325-328. |
| [2] | Richert L,Variola F,Rosei F,et al.Adsorption of proteins on nanoporous Ti surfaces.Surf.Sci.,2010,604(7):1445-1451. |
| [3] | 武楠,冯波,鲁雄,等.有机磷酸酯改性钛表面的蛋白质吸附行为.复合材料学报,2008,25(6):63-66. |
| [4] | Wei Z,Huang W,Hou G,et al.Studies on adsorption isotherms of endotoxin and BSA using an affinity column.Process.Biochem.,2007,42(2):285-287. |
| [5] | Simi C K,Abraham E.Nanocomposite based on modified TiO2-BSA for functional.applications.Colloid.Surf.B:Biointerface.,2009,71(8):319-324. |
| [6] | Feng B,Weng J,Yang B C,et al.Surface characterization of titanium and adsorption of bovine serum albumin.Mater.Character.,2003,49(2):129-137. |
| [7] | Nakanishi K,Sakiyama T,Imamura K.On the adsorption of proteins on solid surfaces,a common but very complicated phenomenon.J.Blosci.Bioeng.,2001,91(3):233-244. |
| [8] | Feng B,Chu X J,Chen J M,et al.Hydroxyapatite coating on titanium surface with titania nanotube layer and its bond strength to substrate.J.Por.Mater.,2010,17(4):453-458. |
| [9] | Gao L,Feng B.Wang J X,et al.Micro/nanostructural porous surface on titanium and bioactivity.J.Biomed.Mater.Res.B:Appl.Biomater.,2009,89B (2):335-341. |
| [10] | Narasimhan B,Peppas N.Molecular analysis of drug delivery systems controlled by dissolution of the polymer carrier.J.Pharm.Sci.,1997,86(1):297-304. |
| [11] | Siepmann J,Podual K,Sriwongjanya M,et al..A new model describing the swelling and drug release kinetics from hydroxypropyl methylcellulose tablets.J.Pharm.Sci.,1999,88(1):65-72. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%