利用超声探针在铝合金圆片上雕刻微米沟槽图案作为模板,并成功地将此沟槽图案转移到羟基磷灰石(HAP)陶瓷表面上.扫描电子显微镜(SEM)表征结果显示3种沟槽宽度分别为20、40和60μm,与预先设计沟槽的尺寸一致；接触角测量结果表明,沟槽化HAP表面的纯水润湿性随沟槽宽度的减小而明显提高.对体外培养在三种沟槽尺寸HAP表面成骨细胞(MC3T3-E1)的形态观察、细胞核染色计数以及细胞取向角测量统计分析的结果表明,沟槽化的HAP表面具有引导成骨细胞沿沟槽方向铺展的作用,而且这种引导作用随着沟槽变窄而显著增强,但这种引导作用并没有对成骨细胞的早期(30 h)增殖行为产生影响.
参考文献
| [1] | Yuan H P,De Bruijn J D,Li Y,et al.Bone formation induced by calcium phosphate ceramics in soft tissue of dogs:a comparative study between porous alpha-TCP and beta-TCP.J.Mater.Sci.Mater.Med.,2001,12(1):7-13. |
| [2] | Yuan H P,Kurashina K,de Bruijn J D,et al.A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics.Biomaterials,1999,20(19):1799-1806. |
| [3] | Li Y,Tjandra W,Tam K C.Synthesis and characterization of nanoporous hydroxyapatite using cation surfactants as templates.Mater.Res.Bull.,2008,43(8/9):2318-2326. |
| [4] | Hornez J C,Chai F,Monchau F,et al.Biological and physicochemical assessment of hydroxyapatite (HA) with different porosity.Biomolecular Engineering,2007,24(5):505-509. |
| [5] | Karageorgiou V,Kaplan D.Porosity of 3D biomaterial scaffolds and osteogenesis.Biomaterials,2005,26(27):5474-5491. |
| [6] | Jones A C,Arns C H,Hutmacher D W,et al.The correlation of pore morphology,interconnectivity and physical properties of 3D ceramic scaffolds with bone ingrowth.Biomaterials,2009,30(7):1440-1451. |
| [7] | Dunn G A,Heath J P.A new hypothesis of contact guidance in tissue cells.Exp.Cell Res.,1976,101(1):1-14. |
| [8] | Rovensky Y A,Slavnaja I L,Vasiliev J M.Behavior of fibroblast-like cells on grooved surfaces.Exp.Cell Res.,1971,65(1):193-201. |
| [9] | Chaubey A,Ross K J,Leadbetter R M,et al.Surface patterning:tool to modulate stem cell differentiation in an adipose system.J.Biomed.Mater.Res.B,2008,84B(1):70-78. |
| [10] | Yim E K E Pang S W,Leong K W.Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage.Exp.Cell Res.,2007,313(9):1820-1829. |
| [11] | Tzvetkova-Chevolleau T,Stephanou A,Fuard D,et al.The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure.Biomaterials,2008,29(10):1541-1551. |
| [12] | Gerecht,S,Bettinger,C J,Zhang,Z,et al.The effect of actin disrupting agents on contact guidance of human embryonic stem cells.Biomaterials,2007,28(28):4068-4077. |
| [13] | Kunzler,T P,Huwiler,C,Drobek,T,et al.Systematic study of osteoblast response to nanotopography by means of nanoparticledensity gradients.Biomaterials,2007,28(33):5000-5006. |
| [14] | Lu X,Leng Y.Quantitative analysis of osteoblast behavior on microgrooved hydroxyapatite and titanium substrata.J.Biomed.Mater.Res.A,2003,66A(3):677-687. |
| [15] | Lu X,Leng Y.Comparison of the osteoblast and myoblast behavior on hydroxyapatite microgrooves.J.Biomed.Mater.Res.B,2009,90B(1):438-445. |
| [16] | Xia Y,Whitesides G M.Soft lithography.Annu.Rev.Mater Sci.,1998,28(1):153-184. |
| [17] | Holthaus M,Rezwan K.Comparison of Three Microstructure Fabrication Methods for Bone Cell Growth Studies.ASME Conf.Proc.,Evanston,2008:483-490. |
| [18] | Holthaus M,Twardy S,Stolle J,et al.Micromachining of ceramic surfaces:hydroxyapatite and zirconia.J.Mater.Process.Tech.,2012,212(3):614-624. |
| [19] | Berger J,Grosse H M,Pistillo N,et al.Ultraviolet laser interference patterning of hydroxyapatite surfaces.Appl.Surf.Sci.,2011,257(7):3081-3087. |
| [20] | Huang L Q,Zhao J W,Wang Y C,et al.Preparation and study of ordered porous anodic alumina film.Journal of Xi'an Jiaotong University,2003,37(10):1094-1097. |
| [21] | Wu D,Chen Q D,Yao Jet al.A simple strategy to realize biomimetic surfaces with controlled anisotropic wetting.Appl.Phys.Len.,2010,96(5):053704-1-3. |
| [22] | Zhu L,Feng Y Y,Ye X Y,et al.Study on controllable wettability of roughness surfaces.Chinese Journal of Sensors and Actuators,2006,19(5):1709-1712. |
| [23] | Wenzel Robert N.Resistance of solid surfaces to wetting by water.Ind.Eng.Chem.,1936,28(8):988-994. |
| [24] | Wenzel R N.Surface roughness and contact angle.J.Phys.Chem.,1949,53(9):1466-1467. |
| [25] | Cui X S,Yao X,Liu H H,et al.Superhydrophobic surfaces:design and fabrication of micro/nanomicrotextures and tuning of wetting behavior.Materials China,2009,28(12):41-52. |
| [26] | Brunette D M.Fibroblasts on micromachined substrata orient hierarchically to grooves of different dimension.Exp.Cell.Res.,1986,164(1):11-26. |
| [27] | McKee C T,Raghunathan V K,Nealey P F,et al.Topographic modulation of the orientation and shape of cell nuclei and their influence on the measured elastic modulus of epithelial cells.Biophys.J.,2011,101(9):2139-2146. |
| [28] | Nelson C M,Chen C S.Cell-cell signaling by direct contact increases cell proliferation via a PI3K-dependent signal.FEBS Letters,2002,514(2/3):238-242. |
| [29] | Roca-Cusachs P,Alcaraz J,Sunyer R,et al.Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation.Biophys.J.,2008,94(12):4984-4995. |
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