开发了以核苷酸锆纳米颗粒为载体固定化脂肪酶的方法,考察了固定化时间、缓冲液pH值及浓度和酶载量等因素对固定化酶在有机相中催化烯丙醇酮酯交换反应性能的影响.结果表明,一元尿苷酸锆(粒径30～50nm)是较佳的载体,在磷酸缓冲液pH=8.0和浓度为0.03mol/L时,酶与载体质量比为4,固定化6h制得的固定化酶效果最佳.固定化酶的活力回收率是游离酶的6.8倍;对映体选择率(E=200)明显高于游离酶(E=86).以一元尿苷酸锆作为载体制得的固定化酶的操作稳定性显著提高,重复利用20批次后残余活力为58.7%,而游离酶仅为4%.
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