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在水/有机溶剂双相体系中,利用重组大肠杆菌DM206 [pET28a-trpBA/BL21 (DE3)]色氨酸合成酶催化L-丝氨酸和吲哚合成了L-色氨酸.考察了有机溶剂、有机相乙酸乙酯体积分数、反应温度、水相pH、底物L-丝氨酸与吲哚摩尔比、底物L-丝氨酸浓度、表面活性剂和酶添加量对色氨酸合成酶酶活的影响.结果表明,酶法最佳转化条件为:有机相溶剂为乙酸乙酯,乙酸乙酯的体积分数为2.5%,反应温度为35℃,水相pH值为8,底物L-丝氨酸与吲哚的摩尔比为1∶1.2,底物L-丝氨酸浓度为100mmol/L,吐温80的质量分数为0.4%,反应介质中酶的添加量为20g/L.在此条件下酶促反应2.5 h,L-丝氨酸转化率可达95%.

参考文献

[1] Leuchtenberger W;Huthmacher K;Drauz K .Biotechnological production of amino acids and derivatives: current status and prospects[J].Applied Microbiology and Biotechnology,2005(1):1-8.
[2] 程立坤,赵春光,黄静,徐庆阳,谢希贤,陈宁.葡萄糖浓度对大肠杆菌发酵L-色氨酸的影响[J].食品与发酵工业,2010(03):5-9.
[3] 于金龙,王静,李剑欣,郭长江,黄英武,徐琪寿.大肠杆菌色氨酸生物合成途径关键酶的调控研究[J].生物工程学报,2008(05):844-850.
[4] 李鑫,刘军,赵沁沁,徐爱才.共表达SHMT和Tpase载体的构建及双酶法合成L-色氨酸[J].生物工程学报,2010(09):1302-1308.
[5] 庞敏,王海磊,姚建铭,李俊,马云峰.以吲哚和DL-丝氨酸为底物固定化酶法合成L-色氨酸[J].食品与发酵工业,2008(10):6-9.
[6] 张绪梅,郭长江,刘云,杨继军,韦京豫,徐琪寿.大肠杆菌trpBA基因的克隆表达[J].生物技术通讯,2006(01):12-14.
[7] Kirchner G;Scollar M P;Klibanov A M .[J].Journal of the American Chemical Society,1985,107:7072.
[8] 皮晓栋,周娅芬,周丽梅,袁茂林,李瑞祥,付海燕,陈华.阳离子表面活性剂存在下水/有机两相体系中双环戊二烯氢甲酰化[J].催化学报,2011(04):566-571.
[9] Schewe, H.;Holtmann, D.;Schrader, J. .P450_(BM-3)-catalyzed whole-cell biotransformation of α-pinene with recombinant Escherichia coli in an aqueous-organic two-phase system[J].Applied Microbiology and Biotechnology,2009(5):849-857.
[10] Gunawan C;Breuer M;Hauer B;Rogers PL;Rosche B .Improved (R)-phenylacetylcarbinol production with Candida utilis pyruvate decarboxylase at decreased organic to aqueous phase volume ratios[J].Biotechnology Letters,2008(2):281-286.
[11] Hildebrand F;Lutz S .Electroenzymatic synthesis of chiral alcohols in an aqueous - organic two-phase system[J].Tetrahedron, Asymmetry: The International Journal for Repid Publication on all Aspects of Asymmetry in Orgainc, Inorganic, Organometallic, Physical and Bio-Organic Chemistry,2007(10):1187-1193.
[12] Foresti ML;Pedemera A;Ferreira ML;Bucala V .Kinetic modeling of enzymatic ethyl oleate synthesis carried out in biphasic systems[J].Applied Catalysis, A. General: An International Journal Devoted to Catalytic Science and Its Applications,2008(1/2):65-72.
[13] 刘森林,宗敏华,涂然,周番.水/有机溶剂双相中杏仁醇腈酶促不对称合成(R)-苯乙氰醇[J].催化学报,2001(01):67-70.
[14] Rosa, PAJ;Ferreira, IF;Azevedo, AM;Aires-Barros, MR .Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals[J].Journal of chromatography, A: Including electrophoresis and other separation methods,2010(16):2296-2305.
[15] 刘森林,宗敏华.水/有机溶剂双相中郁李醇腈酶催化含硅(R)-酮氰醇对映选择性合成[J].催化学报,2006(01):25-28.
[16] Wei D Z;Zhu J H;Cao X J .[J].Biochemical Engineering Journal,2002,11:95.
[17] 吴金川,Hayashi Y,Talukder M.M.R.反胶团酶催化研究新进展[J].化学工程,2002(06):41-43.
[18] 肖俊霞,段明峰,吴卫霞,何涛.超临界流体中的酶催化[J].精细石油化工进展,2003(05):44-46.
[19] 张飞,熊吉滨,刘均忠,刘鹏刚,焦庆才.乙酰鸟氨酸脱乙酰酶固定化细胞拆分D,L-缬氨酸[J].高等学校化学学报,2009(08):1577-1580.
[20] Laane C;Boeren S;Vos K;Veeger C .[J].Biotechnology and Bioengineering,1987,30:81.
[21] 韦平和,吴梧桐,王旻.色氨酸酶基因工程菌酶法合成L-色氨酸[J].中国现代应用药学,1999(06):37-39.
[22] Cabrera Z;Palomo J M;Fernandez-Lorente G;Fernandez-Lorente R Guisan J M .[J].Enzyme and Microbial Technology,2007,40:1280.
[23] 刘幽燕,许建和,胡英.吐温-80对固定化脂肪酶催化酮基布洛芬氯乙酯对映体选择性水解反应的影响[J].催化学报,1999(06):667-670.
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