赖氨酸与1,2-萘醌-4-磺酸钠在碱性条件下发生亲核取代反应,生成红褐色的产物,在该体系中加入甲醇后发生褪色反应,褪色最大波长λ_(max)=559 nm,甲醇质量浓度在0.050～7.61 g/L范围内与吸光度降低程度呈良好线性关系,线性回归方程为A=-0.005 17-0.086 79c(g/L),线性相关系数r=0.999 6,检测限为49.5 mg/L,平均回收率为99.1%～102.6%.用于甲醇样品测定,结果满意.
A simple decolouring spectrophotometric method for the determination of methanol was developed in this work. A russety product can be formed by the reaction between Lysine(Lys) and sodium 1,2-naphthoquinone-4-sulfonate(NQS) in an alkaline solution. When methanol was added to the solution, the system involves decolouring process. Beer's law is held in a range of methanol concentration of 0.050～7.61 g/L at a maximal decolouring wavelength of 559 nm. The equation of linear regression is A=-0.00517-0.086 79c(g/L), with a linearly correlation coefficient of 0.999 6. The detection limit of methanol is 49.5 mg/L(3σ) and the recovery rate is in a range of 99.1%～102.6%. Analytical results obtained with this method are satisfactory.
参考文献
| [1] | Boos R N,Merck & Co Inc,Rahway N.J Anal Chem[J],1948,20(10):964 |
| [2] | Wang M L,Wang J T,Choong Y M.Food Compos Anal[J],2004,17(2):187 |
| [3] | Chen S H,Wu H L,Yen C H,Wu S M,Lin S J,Kou H S.J Chromatogr A[J],1998,799(1-2):93 |
| [4] | Garcia de Maria C,Manzano T,Duarte R,Alonso A.Anal Chim Acta[J],1995,309(1-3):241 |
| [5] | Wu M C,Jiang C M,Ho Y Y,Shen S C,Chang H M.Food Chem[J],2007,100(1):412 |
| [6] | Perez-Ponce A,Rambla F J,Garrigues J M,Garrigues S,Guardia de la M.Analyst[J],1998,123(6):1 253 |
| [7] | Savary B J.J Chromatogr A[J],2003,1 017(1-2):151 |
| [8] | HU Zhi-De(胡之德),ZHAO Zheng-Feng(赵正风).Spectrophotometric Method(分光光度法)[M].Yinchuan(银川):Ningxia Pepole Press(宁夏人民出版社),1987:135 |
| [9] | Li Q M,Zhang T T.Spectrochim Acta Part A[J],2007,67:360 |
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