用蒸馏水溶解滇桂艾纳香浸膏,从中提取多糖,通过膜分离、三氯乙酸法脱蛋白、二乙氨基乙基(DEAE)纤维素脱色、SephadexG10、SephadexG50 凝胶柱色谱纯化,获得滇桂艾纳香水溶性多糖(BRP-B).由凝胶渗透色谱(HPGPC)确定 BRP-B 为相对分子质量分布均一的多糖,其数均分子量和质均分子量分别为2654 和 2716 Da.以血浆复钙时间(PRT)为指标,研究 BRP-B 的凝血活性,结果显示 BRP-B 质量浓度为6.25×10-2 g/L时,凝血时间为 248.52 s,凝血时间抑制率达 22.42%.小白鼠离体子宫实验结果显示,BRP-B质量浓度为1.67×10-2、1.97×10-2 及 2.85×10-2 g/L 时能明显增加子宫平滑肌的收缩频率.
参考文献
| [1] | LING Rong.Flora Repubulicae Popularis Sinicae[M].Beijing:Science Press,1979:13-14(in Chinese).林镕.中国植物志[M].北京:科学出版社,1979:13-14. |
| [2] | CAO Jiaqing,DANG Quan,FU Hongwei.Isolation and Identification of Chemical Constituents from Blumea ripa ria DC.[J].J Shenyang Pharmaceut Univ,2007,24(10):615-618(in Chinese).曹家庆,党权,付红伟.滇桂艾纳香化学成分的分离与鉴定[J].沈阳药科大学学报,2007,24(10):615-618. |
| [3] | LEI Ting,LIN Cuiwu,CHEN Haiyan,et al.Determination of Chlorogenic Acid in Blumea riparia(Bl.)DC.by HPLC[J].Lishizhen Med Mater Med Res,2008,19(9):2073-2074(in Chinese).雷婷,林翠梧,陈海燕,等.反相高效液相色谱法测定滇桂艾纳香中绿原酸的含量[J].时珍国医国药,2008,19(9):2073-2074. |
| [4] | ZHENG Dan,ZHANG Xiaoqi,WANG Ying.Chemical Constituents of the Aerial Parts of Blumea riparia[J].Chinese J Nat Med,2007,5(6):421-424(in Chinese).郑丹,张晓琦,王英.滇桂艾纳香地上部分的化学成分[J].中国天然药物,2007,5(6):421-424. |
| [5] | Sun L Q,Wang C H,Shi Q J,et al.Preparation of Different Molecular Weight Polysaccharides from Purphyridium Cruentum and Their Antioxidant Activities[J].Int J Biol Macromol,2009,45:42-47. |
| [6] | Liu W,Wang H Y,Pang X B,et al.Characterization and Antioxidant Activity of Two Low-molecular-weight Polysaccharides Purified from the Fruiting Bodies of Ganoderma Lucidum[J].Int J Biol Macromol,2010,46:451-457 |
| [7] | ZHANG Fanhua,YAN Lang,ZHANG Shuming,et al.Structure Elucidation of Low Molecular Weight Pumpkin Polysaccharides LWPP-Ia[J].Food Sci,2009,30(1):25-27(in Chinese).张凡华,严浪,张树明,等.低分子量南瓜多糖LWPP-Ia 的结构研究[J].食品科学,2009,30(1):25-27. |
| [8] | TIAN Limei,WANG Man.Studies on Extraction,Isolation and Composition of Lycium Barbarum Plysaccharides[J].China J Chinese Mater Med,2006,3(19):1603-1608(in Chinese).田丽梅,王曼.枸杞多糖的提取分离和其组成研究[J].中国中药杂志,2006,3(19):1603-1608. |
| [9] | LI Xialan,WEI Guodong,WANG Zhaojing,et al.Purification and Physico-chemical Characteristics of Polysaccharide from leaf Mustard Isolated with Dilute Alkali[J].Chinese J Appl Chem,2006,23(11):1304-1306(in Chinese).李夏兰,魏国栋,王昭晶,等.碱提芥菜多糖ALP-2的分离纯化及理化性质分析[J].应用化学,2006,23(11):1304-1306. |
| [10] | ZHANG Weijie.The Technology of Biochemical Study of Glycoconjugates[M].Hangzhou:Zhejiang University Press,1999:112-117,193-198(in Chinese).张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,1999:112-117,193-198.. |
| [11] | Yu Yan,WU Ning,WANG Xiuxia.Influence of Shenghuazhixueying on autonomic movement of Isolated Uterine from Rats[J].Chinese J Tradit Med Sci Technol,2003,10(2):93-93(in Chinese).于燕,吴宁,王秀霞.生化止血饮对大鼠离体子宫自发运动影响的实验研究[J].中国中医药科技,2003,10(2):93-93 |
| [12] | JIANG Weixin,ZHAO Xi.The Anti-coagulant Effect of Polysaccharides from Long Dan[J].Heilongjiang Med.J,2008,21(5):31-32(in Chinese).江蔚新,赵玺.龙胆多糖的抗凝血作用的研究[J].黑龙江医药,2008,21(5):31-32. |
| [13] | LIU Guoyun,CHI Xiaoli,MA Yuan,et al.Effect of DSS-A-N-30,An Active Ingredient of Danggui Shaoyao San,on Contractility of Rat Uterine Smooth Muscle In Vitro[J].Chinese J Pharmacol Toxicol,2007,21(5):427-433(in Chinese).刘国云,迟晓丽,马渊,等.当归芍药散活性部位 DSS-A-N-30 对大鼠离体子宫平滑肌收缩的影响[J].中国药理学与毒理学杂志,2007,21(5):427-433. |
| [14] | SHI Xubo,HU Dayi.Coagulation Mechanism and New Concepts of Anticoagulant Therapy[J].Clin Focus,2008,23(22):1597-1599(in Chinees).史旭波,胡大一.凝血机制与抗凝治疗新观念[J].临床汇萃,2008,23(22):1597-1599. |
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