制备了能在水溶液中长时间稳定存在的十六烷基三甲基溴化铵/山梨醇酐硬脂酸酯(CTAB/Span-60)微乳液凝胶(MBG),确定了Span-60在乳化剂EM(正丁醇与Span-60的混合物)中的质量分数范围;分别以正己酸与正己醇的酯化反应、α-单硬脂酸甘油酯的水解反应、消旋布洛芬与正辛醇的不对称酯化反应为指示反应,研究了CTAB,/Span-60 MBG固定化脂肪酶的催化活性及立体选择性.结果表明,Span-60在EM中的质量分数小于57%时可形成机械强度较好的CTAB/Span-60 MBG;其固定化脂肪酶在有机溶剂中的酯化活性随EM中Span-60含量的增加先是逐渐增大,35%时最大,后又逐渐小幅度降低,在所考察的Span-60含量范围内均比在CTAB MBG中高;在水溶液中固定化脂肪酶能顺利催化α-单硬脂酸甘油酯的水解反应,24 h后反应转化率不再随反应时间的延长而增加,其水解活性在重复使用9次后仅降低13.68%,表明CTAB/Span-60MBG固定化脂肪酶能够顺利进行分离并重复使用;此体系的脂肪酶也选择性地催化生成S-构型布洛芬辛酯,产物对映体过量值(eee)随反应的进行缓缓下降,但降幅不大,即其立体选择性要比在CTAB MBG中高.因此,CTAB/Span-60 MBG作为脂肪酶固定化载体既可用于有机溶剂中又可用于水溶液中的生物合成与生物转化反应,扩大了微乳液凝胶固定化脂肪酶的应用范围.
参考文献
| [1] | Haering G,Luisi P L.Hydrocarbon Gels from Water-in-oil Microemulsions[J].J Phys Chem,1986,90(22):5892-5895. |
| [2] | Capitani D.Segre A L.Mobility Measurements in Microemulsion Gels[J].J Phys Chem,1988,92(12):3500-3504. |
| [3] | Rees G D,Nascimento M G,Jenta T R J,et al.Reverse Enzyme Synthesis in Microemulsion-based Organo-gels[J].Biochem Biophys Acta,1991,1073:493-501. |
| [4] | Rees G D,Nascimento M G,Catauro M,et al.Use of Water-in-oil Microemulsion and Gelatin-containing Microemulsionbased Gels for Iipase-catalysed Ester Synthesis in Organic Solvents[J].Indian J Chem,1993,32B(1):30-34. |
| [5] | Fadnavis N W,Koteshwar K.An Unusual Reversible Sol-gel Transition Phenomenon in Organogels and Its Application for Enzyme Immobilization in Gelatin membranes[J].Biotechnol Prog,1999,15(1):98-104. |
| [6] | Jenta T R,Batts G,Ree G D,et al.Kinetic Studies of Chromobacterium Viscosum Lipase in AOT Water-in-oil Microemulsion and Gelatin Microemulsion-based Organogels[J].Biotechnol Bioeng,1997,54:416-427. |
| [7] | Soni K,Madamwar D.Ester Synthesis by Lipase Immobilized on Silica and Microemulsion Based Organogels(MBGs)[J].Process Biochem,2001,36:607-611. |
| [8] | Backlund S,Eriksson K,Kanerva L T,et al.Selective Enzymatic Reactions Using Microemulsion-based Gels[J].Colloids Surf B Biointerf,1995,4:121-127. |
| [9] | Blattner C,Zoumpanioti M,Kr(o)nerJ,et al.Biocatalysis Using Lipase Encapsulated in Microemulsion-based Organogels in Supercritical Carbon Dioxide[J].J Supercrit Fluids,2006,36:182-193. |
| [10] | Lopez F,Venditti F,Cinelli G,et al.The Novel Hexadecyl Trimethyl Ammonium Bromide(CTAB) Based Organogel as Reactor for Ester Synthesis by Entrapped Candida Rugosa Lipase[J].Process Biochem,2006,41 (1):114-119. |
| [11] | SONG Shaofang,HUANG Xirong,ZHANG Wenjuan,et al.Enantiomenc Separation and Application of Ibuprofen and Its n-Octyl Ester by Chiral HPLC[J].Chinese J Pharm Anal,2002,22(1):54-56(in Chinese).宋少芳,黄锡荣,张文娟,等.布洛芬及其正辛酯对映体的手性高效液相色谱法拆分与应用[J].药物分析杂志,2002,22(1):54-56. |
| [12] | Song S F,Luan Y X,Lu F S.Activity and Enantioselectivity of Lipase Immobilized in CTAB Microemulsion-based Gels[J].Chem Res Chinese Vniv,2007,23 (6):749-752. |
| [13] | Song S F,Luan Y X,Su X R.Study on Manganese Peroxidase Immobilized in Gelatin-containing Microemulsion-based Gels[J].Chem Res Chinese Univ,2005,21 (4):423-425. |
| [14] | SONG Shaofang,HUANG Xirong.Study on The Stereoselectivity of The Immobilized Lepase in Microemulsion Based Gels[J].J Shandong Agric Univ(Nat Sci),2003,34(4):519-522(in Chinese).宋少芳,黄锡荣.微乳液凝胶固定化脂肪酶立体选择性研究[J].山东农业大学学报(自然科学版),2003,34(4):519-522. |
| [15] | SUN Junshe.Enzyme,Enzyme Engineering and Applications[M].Beijing; Chemical Industry Press,2006:275 (in Chinese).孙君社.酶与酶工程及其应用[M].北京:化学工业出版社,2006:275. |
| [16] | Backlund S,Eriksson F,Karlsson S,et al.Enzymatic Esterification and Phase Behavior in Ionic Microemulsions with Different Alcohols[J].Colloid Polym Sci,1995,273(6):533-538. |
| [17] | XU Yanli.Functions of Surfactants[M].Beijing:Chemical Industry Press,2000:68(in Chinese).徐燕莉.表面活性剂的功能[M].北京:化学工业出版社,2000:68. |
| [18] | Adams S S,Bresloff P,Mason G C.Pharmacological Difference Between the Optical Isomers of Ibuprofen:Evidence for Metabolic Inversion of the(-)Isomer[J].J Pharm Pharmacol,1976,28:156-159. |
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