以罗丹明B为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合法制备了罗丹明B分子印迹聚合物(MIP)微球,并用扫描电子显微镜表征.采用紫外分光光度法测定了印迹分子罗丹明B与功能单体丙烯酰胺二者之间的结合常数(K=5.303 × 10(3)(mol/L)(-1))和化学计量比(n=1).考察了沉淀剂的种类和用量对聚合物微球的影响.将分子印迹聚合物微球应用于固相萃取材料自制固相萃取柱,从加标罗丹明B的红椒粉中萃取罗丹明B.本文优化了固相萃取条件,高效液相色谱检测表明,在一定的萃取条件下,分子印迹聚合物对加标量为0.479 mg/kg的辣椒中罗丹明B的萃取加标回收率可达91.7%~103.5%.
参考文献
[1] | McGregor D B,Brown A G,Howgate S,et al.Responses of the L5178Y Mouse Lymphoma Cell Forward Mutation Assay.Ⅴ:27 Coded Chemicals[J].Environ Mol Mutagen,1991,17:196-219. |
[2] | Amut E,Fu Q,Fang Q,et al.In Situ Polymerization Preparation of Chiral Molecular Imprinting Polymers Monolithic Column for Amlodipine and Its Recognition Properties Study[J].J Polym Res,2010,17(3):401-409. |
[3] | Ngnyen T H,Ansell R J.Fluorescent Imprinted Polymer Sensors for Chiral Amines[J].Org Biomol Chem,2009,7(6):1211-1220. |
[4] | CHANG Pingping,ZHANG Zhujun.A Novel Chemiluminescence Array Sensor Based on Molecularly Imprinted Polymer[J].Acta Chim Sin,2009,67(23):2727-2731(in Chinese).常平平,章竹君.一种新型分子印迹聚合物基的化学发光阵列传感器[J].化学学报,2009,67(23):2727-2731. |
[5] | Bruggemann O.Catalytically Active Polymers Obtained by Molecular Imprinting and Their Application in Chemical Reaction Engineering[J].Biomol Eng,2001,18(1):1-7. |
[6] | ZHANG Zhaohui,ZHANG Huabin,HU Yufang,et al.Proparation and Characterization of Stereoselective L-Histidine Imprinted Mierospheres[J].Chem J Chinese Univ,2008,29:1941-1946(in Chinese).张朝晖,张华斌,胡宇芳,等.L-组氨酸手性识别印迹固定相的制备及表征[J].高等学校化学学报,2008,29:1941-1946. |
[7] | Baggiani C,Anfossi L,Giovannoli C.Solid Phase Extraction of Food Contaminants Using Molecular Imprinted Polymers[J].Anal Chim Acta,2007,S91(1):29-39. |
[8] | CAO Ximin,LIAO Ling,DU Liming.Recognition Properties of Enoxacin Imprinted Polymer[J].Chinese J Appl Chem,2008,25(1):43-47(in Chinese).曹玺民,廖玲,杜黎明.依诺沙星分子印迹聚合物的分子识别[J].应用化学,2008,25(1):43-47. |
[9] | WANG Sui,WANG Yongzhen,GONG Guoquan,et al.Studies on the Molecular Recognition and Binding Characteristics in Rhodamine B Molecularly Imprinted Polymer[J].J Lanzhou Univ,2003,39(2):57-60(in Chinese).王邃,王永珍,龚国权,等.罗丹明B分子印迹聚合物的分子识别与结合特性的研究[J].兰州大学学报,2003,39(2):57-60. |
[10] | Liu R G,Li X,Li Y Q,et al.Effective Removal of Rhodamine B from Contaminated Water using Non-covalent Imprinted Microspheres Designed by Computational Approach[J].Biosens Bioelectron,2009,25(3):629-634. |
[11] | Yoshimatsu K,Lejeune J,Spivak D A,et al.Peptide-imprinted Polymer Microspheres Prepared by Precipitation Polymerization Using a Single Bi-functional Monomer[J].Analyst,2009,134(4):719-724. |
[12] | DAI Qing,WANG Yan,BAO Xuewei,et al.Preparation and Evaluation of Sudan Red Ⅰ Molecularly Imprinted Polymer[J].Chinese J Chromatogr,2009,27(6):764-768(in Chinese).戴晴,王妍,包学伟,等.苏丹红Ⅰ分子印迹聚合物的制备及其性能评价[J].色谱,2009,27(6):764-768. |
[13] | RONG Fei,LI Ping,YUAN Chunwei.Preparation of Molecularly Imprinted Microspheres Using L-2-Chloromandelic Acid as Template by Precipitation Polymerization[J].J Funct Polym,2005,18(4):607-612(in Chinese).戎非,李萍,袁春伟.沉淀聚合法制备右旋邻氯扁桃酸分子印迹聚合物微球[J].功能高分子学报,2005,18(4):607-612. |
[14] | Naka Y,Kaetsu I,Yamamoto Y,et al.Preparation of Microspheres by Radiation-induced Polymerization 1:Mechanism for the Formation of Monodisperse Poly(diethylene glycol dimethacrylate)Microspheres[J].J Polym Sci Part A,1991,29(8):1197-1202. |
[15] | Yan S L,Gao Z X,Fang Y J,et al.Characterization and Quality Assessment of Binding Properties of Malachite Green Molecularly Imprinted Polymers Prepared by Precipitation Polymerization in Acetonitrile[J].Dyes Pigm,2007,74(3):572-577. |
[16] | LU Chunyang,MA Xiangxia,HE Xiwen,et al.Preparation and Selective Permeation Characterization of Molecularly Imprinted Membranes with o-Vanillin as Template[J].Acta Chim Sin,2005,63(6):479-483(in Chinese).卢春阳,马向霞,何锡文,等.邻香草醛分子印迹聚合物膜的制备及其透过选择性质的研究[J].化学学报,2005,63(6):479-483. |
[17] | Lasakova M,Jandera P.Molecularly Imprinted Polymers and Their Application in Solid Phase Extraction[J].J Sep Sci,2009,32(5/6):799-812. |
[18] | MENG Fanping,LIU Jiao.Molecularly Imprinted Polymers and Their Application to Solid-Phase Extraction[J].Period Ocean Univ China,2008,38(2):237-243(in Chinese).孟范平,刘娇.分子印迹聚合物及其在固相萃取中的应用[J].中国海洋大学学报,2008,38(2):237-243. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分:
-
10%
-
20%
-
30%
-
40%
-
50%