在Tris-HCl缓冲溶液体系中(pH=7.4),白藜芦醇与人血清白蛋白相互作用,对蛋白的内源性荧光产生猝灭作用,而且,同步荧光的强度与人血清白蛋白的浓度成正比.基于此,建立了以白藜芦醇为荧光探针,运用固定波长同步荧光光谱法测定生物样品中蛋白质含量的新方法.体系同步荧光光谱特征及强度受△λ、反应介质和离子强度等因素的影响.在最佳实验条件下,体系的同步荧光强度(I)与人血清白蛋白在1_380～276.0 mg/L的浓度范围内呈良好的线性关系,检测限为1.037 mg/L(n=11).对血清、尿样和唾液等生物样品进行测定,回收率在93.5%～105.8%之间,与传统的考马斯亮蓝(G-250)法作对照,结果一致.
参考文献
| [1] | Jiangsu New Medical College.Dictionary of Chinese Materia Medica[M].Shanghai:Shanghai Scientific and Technological Publishing House,1985 (in Chinese).江苏新医学院编.中药大辞典[M].上海:上海科学技术出版社,1985. |
| [2] | Jang M,Cai L,Udeani G O,et al.Cancer Chemopreventive Activity of Resveratrol,a Natural Product Derived from Gropes[J].Science,1997,275(5297):218-220. |
| [3] | He X M,Carter D C.Atomic Structure and Chemistry of Human Serum Albumin[J].Nature,1992,358(6383):209-215. |
| [4] | ZHANG Gencheng,XU Jieyan.Interaction Between Chromium(Ⅵ) and Bovine Serum Albumin[J].Chinese J Appl Chem,2010,27(2):191-196(in Chinese).张根成,许洁艳.Cr(Ⅵ)与牛血清白蛋白的相互作用[J].应用化学,2010,27(2):191-196. |
| [5] | CHI Yanhua,ZHUANG Jia,BI Xinying,et al.Interaction of Diethylmalonylurea and Bovine Serum Albumin by Fluorescence Method[J].Chinese J Appl Chem,2007,24(10):1167-1171 (in Chinese).迟燕华,庄稼,毕欣颖,等.二乙基巴比妥酸与牛血清白蛋白相互作用的荧光光谱法[J].应用化学,2007,24(10):1167-1171. |
| [6] | Cui F,Kong X,Qin L,et al.Specific Interaction of 4'-O-(a-1 -Cladinosyl) Daunorubicin with Human Serum Albumin:The Binding Site Ⅱ on HSA Molecular Using Spectroscopy and Modeling[J].J Photochem Photobiol B,2009,95(3):162-169. |
| [7] | Sun W,Jiao K,Wang X,et al.Electrochemical Studies of the Reaction Between Albumin and Amaranth and Its Analytical Application[J].J Electroanal Chem,2005,578:37-43. |
| [8] | Lu L,Zi Y,Wang H.Microdetermination of Human Serum Albumin by Differential Pulse Vohammetry at a 1-Cysteine Modified Silver Electrode[J].J Chem Sci,2008,120(4):419-424. |
| [9] | Cui F L,Cui Y R,Luo H X.The Interaction Between N-n-Undecyl-N'-(sodium p-aminobenzenesulfonate) Thiourea and Serum Albumin Studied Using Various Spectroscopies and the Molecular Modeling Method[J].Biopolymers,2006,83(2):170-181. |
| [10] | Liu R,Yang J,Sun C,et al.Resonance Light-scattering Method for the Determination of BSA and HSA with Sodium Dodecyl Benzene Sulfonate or Sodium Lauryl Sulfate[J].Aral Bioanal Chem,2003,377:375-379. |
| [11] | CHEN Guozhen,HUANG Xianzhi,ZHENG Zhuzi.Methods of Fluorescence Analysis[M].Beijing:Science Press,1990:112.(in Chinese).陈国珍,黄贤智,郑朱梓.荧光分析法[M].北京:科学出版社,1990:112. |
| [12] | YAN Yuan,PENG Xuejung,XU Jingou,et al.Application of Convolution Curve Method to Synchronous Fluorimetric Method for the Simultaneous Determination of Phenol and p-Dihydroxybenzene[J].J Instrum Anal,1995,14(1):1-4(in Chinese).鄢远,彭学军,许金钩,等.褶合曲线分析法用于同步荧光光谱法同时测定苯酚和对苯二酚[J].分析测试学报,1995,14(1):1-4. |
| [13] | WU Qiuhna,ZHOU Xin,ZHANG Xiaohuan,et al.Spectroscopic Study on the Interaction Between Resveratrol and Human Serum Albumin[J].Spectrosc Spectr Anal,2009,29(1):226-230(in Chinese).吴秋华.周欣,藏晓欢,等.白藜芦醇与人血清白蛋白相互作用的光谱研究[J].光谱学与光谱分析,2009,29(1):226-230. |
| [14] | Zhang Y,Zhou B,Zhang X,et al.Interaction of Malachite Green with Bovine Serum Albumin:Determination of the Binding Mechanism and Binding Site by Spectroscopic Methods[J].J Hazard Mater,2009,163:1345-1352. |
| [15] | Zhang H,Wang Y,Jiang M.A Fluorimetric Study of the Interaction of C.I.Solvent Red 24 with Haemoglobin[J].Dyes Pigm,2009,82:156-163. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%