采用光谱相关色谱法对延胡索药材指纹图谱中的有效组分延胡索乙素峰进行确认；利用多元曲线分辨-交替最小二乘法(MCR-ALS)对延胡索乙素重叠峰簇进行解析,得到延胡索乙素的真实色谱峰,其光谱还原率大于0.999,并对其进行准确定量,该方法可作为指纹图谱的辅助方法,为中药的真伪鉴定和质量客观评价提供可靠依据；最后讨论了药材的炮制对延胡索乙素含量的影响.
参考文献
| [1] | HE Kai,GAO Jianli,ZHAO Guangshui.Advances in Studies on Chemistry,Pharmacology,and Quality Control of Corydalis Yanhusuo[J].Chinese Trad Herb Drugs,2007,38(12):1909-1912(in Chinese).贺凯,高建莉,赵光树.延胡索化学成分、药理作用及质量控制研究进展[J].中草药,2007,38(12):1909-1912. |
| [2] | WANG Xiaoling,ZHENG Zhen,HONG Zhanying,et al.Advancements on Chemical Components and Quality Control of Rhizoma Corydalis[J].Lishizhen Med Mater Med Res,2011,22(1):227-229(in Chinese).王晓玲,郑振,洪战英,等.中药延胡索的化学成分与质量控制研究进展[J].时珍国医国药,2011,22(1):227-229. |
| [3] | XIE Caijuan,ZHANG Zhiqi,ZHANG Fuqiang,et al.RP-HPLC Simultaneous Determination of Tetrahydropalmatine and Protopine in Rhizoma Corydalis[J].J Shaanxi Normal Univ (Nat Sci Ed),2005,31 (1):82-85 (in Chinese).谢彩娟,张志琪,张富强,等.RP-HPLC法同时测定延胡索药材中延胡索乙素和原阿片碱[J].陕西师范大学学报(自然版),2005,33(1):82-85. |
| [4] | CHENG Xu,LU Tulin,ZHANG Xianhong.Determination of Tetrahydropalmatine of Different Processed Products of Rhizoma Corydalis by HPLC[J].Chinese Trad Pat Med,2003,25(9):726-727(in Chinese).陈旭,陆兔林,张先洪.HPLC测定延胡索不同炮制品中延胡索乙素含量[J].中成药,2003,25(9):726-727. |
| [5] | LUO Guoan,WANG Yiming,RAO Yi.Progress of Modernization in Chinese Traditional Medicine and Patent Medicine[J].Chinese Trad Pat Med,2000,22(1):71-79(in Chinese).罗国安,王义明,饶毅.中药中成药现代化进程[J].中成药,2000,22(1):71-79. |
| [6] | XIE Peishan.Study and Application of Chromatographic Fingerprinting Quality Control Mode on Chinese Herbal Medication[J].World Sci Technol/Mod Trad Chinese Med,2001,3(6):28-32(in Chinese).谢培山.中药色谱指纹图谱质量控制模式的研究和应用[J].世界科学技术—中药现代化,2001,3(6):28-32. |
| [7] | XIE Caijuan,ZHANG Zhiqi.HPLC Fingerprint of Corydalis Yanhusuo[J].Chinese J Pharm Anal,2004,24(supp):87-89(in Chinese).谢彩娟,张志琪.延胡索药材的HPLC指纹图谱[J].药物分析杂志,2004,24(增刊):87-89. |
| [8] | ZHOU Yuehua,CAO Liu,LI Yingchun,et al.Fingerprint of Rhizoma Corydalis and Processed Corydalis[J].Tianjin Pharm,2010,22 (6):22-27 (in Chinese).周乐华,曹柳,李迎春,等.延胡索鲜品及炮制品的指纹图谱研究[J].天津药学,2010,22(6):22-27. |
| [9] | JIA Zehui,ZHANG Fang,LI Hua.Simultaneous Determination of Camptothecin and Its Derivants Using MCR-ALS with HPLC-DAD[J].Comput Appl Chem,2008,25(2):141-144(in Chinese).贾泽慧,张方,李华.MCR-ALS与HPLC-DAD相结合同时测定喜树碱及其衍生物[J].计算机与应用化学,2008,25(2):141-144. |
| [10] | WANG Kang,JIA Zehui,ZHANG Zhiqi,et al.Peak Shift Correction Used in Resolution of Overlapped HPLC Signals of Traditional Chinese Medicine by Three-way PARAFAC Model[J].Chem J Chinese Univ,2009,30 (2):268-273 (in Chinese).王康,贾泽慧,张志琪,等.谱峰漂移校正技术结合三维平行因子分析方法用于分辨中药重叠高效液相色谱信号[J].高等学校化学学报,2009,30(2):268-273. |
| [11] | WANG Yali,LIANG Yizeng,HU Yun,et al.Comparison Studies of Constituents Between Dangguibuxuetang and Its Single Medicines via Hyphenated Chromatography and Multicomponent Spectral Correlative Chromatography[J].Chem J Chinese Univ,2006,27(2):222-226(in Chinese).王亚丽,梁逸曾,胡芸,等.联用色谱及多组分光谱相关色谱用于当归补血汤与单味药成分的比较研究[J].高等学校化学学报,2006,27(2):222-226. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%