在酸性介质中,钼酸根、钨酸根等同多酸根与盐酸哌唑嗪(PRH)和甲磺酸多沙唑嗪(Dox)等α1受体阻滞剂反应形成离子缔合物时,会导致体系的共振瑞利散射(RRS)显著增强并出现新的RRS光谱,最大散射峰分别位于367 nm(钼酸根体系)和290 nm(钨酸根体系).PRH和Dox与同多酸根的反应产物具有相似的RRS光谱特征.其反应的适宜酸度分别为pH值2.1 ～2.3(钼酸根-PRH体系)和pH值3.1 ～3.3(钨酸根-PRH体系).在一定浓度范围内,不同的反应体系RRS强度增强程度与药物浓度成正比,均可用于痕量药物的测定.反应具有很高的灵敏度,不同同多酸对PRH的检出限(3σ/s)分别为4.76 μg/L(钼酸根-PRH体系)和9.88 μg/L(钨酸根-PRH体系).方法也具有较好的选择性,用于片剂和人尿液中的α1受体阻滞剂的测定,结果满意.此外,本文还应用计算化学软件Gaussview3.07和Gaussian03W,采用密度泛函法,在B3 LYP/6-31G基组水平上计算了盐酸哌唑嗪的电荷分布,对反应机理和RRS增强的原因进行了讨论.
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