在玻碳电极(GCE)表面首先用增敏作用的多壁碳纳米管(MWCNTs)夹心于两层电沉积的铁氰化镍(NiHCF)氧化还原电化学探针之间,然后以金纳米粒子为固定核酸适配体的载体,构建了检测凝血酶的非标记型核酸适配体生物传感器.利用扫描电子显微镜(SEM)对MWCNTs和NiHCF的形貌进行了表征.利用电化学阻抗谱对传感器的组装过程进行了监测,用循环伏安法(CV)和差分脉冲伏安法(DPV)对传感器的电化学行为进行了研究.以铁氰化镍为探针的传感器对凝血酶的检测在1.0 ng/L～1.0 mg/L范围内呈良好的线性关系,相关系数为0.998,检测限为0.2 ng/L(S/N=3).
参考文献
| [1] | Larry G.Oligonucleotides as Research,Diagnostic,and Therapeutic Agents[J].J Biol Chem,1995,270 (23):13581-13584. |
| [2] | Hianik T,Wang J.Electrochemical Aptasensors-Recent Achievements and Perspectives[J].Electroanalysis,2009,21(11):1223-1235. |
| [3] | Lai R Y,Plaxco K W,Heeger A J.Aptamer-Based Electrochemical Detection of Picomolar Platelet-Derived Growth Factor Directly in Blood Serum[J].Anal Chem,2007,79(1):229-233. |
| [4] | Mir M,Vreeke M,Katakis I.Different Strategies to Develop an Electrochemical Thrombin Aptasensor[J].Electrochem Commun,2006,8(3):505-511. |
| [5] | Wang X,Zhang Y,Banks C E,et al.Non-enzymatic Amperometric Glucose Biosensor Based on Nickel Hexacyanoferrate Nanoparticle Film Modified Electrodes[J].Colloid Surf B,2010,78:363-366. |
| [6] | Tsai T H,Chen T W,Chen S M.Selective Electroanalysis of Ascorbic Acid Using a Nickel Hexacyanoferrate and Poly(3,4-ethylenedioxythiophene) Hybrid Film Modified Electrode[J].Electroanalysis,2010,22(14):1655-1662. |
| [7] | Mashhadizadeh M H,Yousefi T,Golikandb A N.A Nickel Hexacyanoferrate and Poly (1-naphthol) Hybrid Film Modified Electrode Used in the Selective Electroanalysis of Dopamine[J].Electrochim Acta,2011,59:321-328. |
| [8] | Wang W,Hao W,Zhang Z,et al.One-step Unipolar Pulse Electrodeposition of Nickel Hexacyanoferrate/Chitosan/Carbon Nanotubes Film and Its Application in Hydrogen Peroxide Sensor[J].Sen Actuat B:Chem,2012,162:353-360. |
| [9] | Lata S,Pundir C S.Fabrication of an Amperometric D-amino Acid Biosensor Based on Nickel Hexacyanoferrate Polypyrrole Hybrid Film Deposited on Glassy Carbon Electrode[J].Bioprocess Biosyst Eng,2013,36:81-89. |
| [10] | Bai L,Yuan R,Chai Y,et al.Highly Sensitive Electrochemical Label-free Aptasensor Based on Dual Electrocatalytic Amplification of Pt AuNPs and HRP[J].Analyst,2011,136:184071845. |
| [11] | YANG Shaoming,ZHA Wenling,LI Hong,et al.Study of Lable-free Bi-analyte Detection Aptamer Biosensor[J].Acta Chim Sin,2013,71:451-456 (in Chinese).杨绍明,查文玲,李红,等.非标记型双底物检测核酸适配体传感器研究[J].化学学报,2013,71:451-456. |
| [12] | De Volder M F L,Tawfick S H,Baughman R H,et al.Carbon Nanotubes:Present and Future Commercial Applications[J].Seience,2013,339:535-539. |
| [13] | Vashist S K,Zheng D,Al Rubeaan K,et al.Advances in Carbon Nanotube Based Electrochemical Sensors for Bioanalytical Applications[J].Biotechnol Adv,2011,29:169-188. |
| [14] | Jacobs C B,Peairs M J,Venton B J.Review:Carbon Nanotube Based Electrochemical Sensors for Biomolecules[J].Anal Chim Acta,2010,662(2):105-127. |
| [15] | Mir M,Jenkins A T A,Katakis I.Ultrasensitive Detection Based on an Aptamer Beacon Electron Transfer Chain[J].Electrochem Commun,2008,10(10):1533-1536. |
| [16] | Frens G.Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions[J].Nat Phys Sci,1973,241:20-22. |
| [17] | Turkevich J,Stevenson P C,Hillier J.A Study of the Nucleation and Growth Processes in the Synthesis of Colloidal Gold[J].Disc Farad Soc,1951,11:55-75. |
| [18] | Chen W,Tang J,Cheng H J,etal.A Simple Method for Fabrication of Sole Composition Nickel Hexacyanoferrate Modified Electrode and Its Application[J].Talanta,2009,80:539-543. |
| [19] | Jayalakshmi M,Gomathi H,Prabhakara Rao G.Investigations on the Electrochemical Behaviour of Prussian Blue Films in Acetonitrile[J].Sol Energ Mat Sol C,1997,45(3):201-209. |
| [20] | Zhao J,Zhang Y,Li H,et al.Ultrasensitive Electrochemical Aptasensor for Thrombin Based on the Amplification of Aptamer-AuNPs-HRP Conjugates[J].Biosens Bioelectron,2011,26:2297-2303. |
| [21] | Xie S,Yuan R,Chai Y,et al.Label-free Electrochemical Aptasensor for Sensitive Thrombin Detection Using Layer-by-layer Self-assembled Multilayers with Toluidine Blue-Graphene Composites and Gold Nanoparticles[J].Talanta,2012,98:7-13. |
| [22] | Liu X R,Li Y,Li J B,et al.Carbon Nanotube-Enhanced Electrochemical Aptasensor for the Detection of Thrombin[J].Talanta,2010,81:1619-1624. |
| [23] | Yuan Y L,Yuan R,Chai Y Q,et al.A Signal-on Electrochemical Probe-Label-Free Aptasensor Using Gold-Platinum Alloy and Stearic Acid as Enhancers[J].Biosens Bioelectron,2010,26:881. |
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