在pH值2.09 ～5.02的Britton-Robinson (BR)缓冲溶液中,头孢曲松钠(CTRX)与吖黄素(AF)形成1∶1的离子缔合物,导致吖黄素溶液荧光猝灭.当分别于最大激发和最大发射(λa/λcm)266 nm/506 rn进行测量时,荧光猝灭值(△F)与CTRX浓度在一定范围呈良好的线性关系.该方法灵敏度高,测定CTRX的线性范围和检出限分别为0.056 ～5.0 mg/L和0.016 mg/L.研究了体系的荧光光谱特征、适宜的反应条件和共存物质的影响,讨论了离子缔合物的组成,基于离子缔合物反应,发展了测定CTRX的高灵敏、简便和快速的新方法,用于血清和尿液中CTRX的测定,结果令人满意.
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