初步考察了水溶液中影响十六烷基三甲基溴化铵(CTAB)/聚乙二醇辛基苯基醚(TX-100)微乳液凝胶(MBG)固定化脂肪酶催化水解消旋布洛芬辛酯的主要因素.结果表明,CTAB/TX-100 MBG固定化脂肪酶在水溶液中能顺利催化水解S-构型布洛芬辛酯生成S-构型布洛芬.随TX-100在EM(正丁醇与TX-100的混合物)中含量的增加,反应转化率(X)逐渐增大,而产物中S-构型布洛芬的对映体过量值(eei)有少许的降低;随凝胶含水量的增加,X及ee;均呈钟罩形变化,二者最大值时对应的凝胶水含量为27.3％,且磷酸缓冲溶液对二者的影响要比溶解明胶的水大;X在反应初期(16 h内)增加明显,随反应时间的继续延长增加缓慢,24h后趋于平衡,而eei随反应时间的延长呈逐渐降低的趋势;随固定化脂肪酶重复使用次数的增多,X在前3次中降幅不大,后几次中降幅逐渐增大,eei则呈逐渐稍微降低的趋势,重复使用10次后,二者分别降低了36.55％和0.52％.
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